TY - JOUR
T1 - A study of different actions of glucanases to modulate microfibrillated cellulose properties
AU - Sharma, Rahul
AU - Garnier, Gil
AU - Haritos, Victoria
N1 - Funding Information:
Open Access funding enabled and organized by CAUL and its Member Institutions. The research leading to these results received funding from the Australian Research Council under grant number IH170100020. The authors have no financial or proprietary interests in any material discussed in this article.
Funding Information:
Funding from the Australian Research Council-Industry Transformation Research Hub Processing Advanced Lignocellulosics (PALS) [grant number IH170100020] is gratefully acknowledged. Many thanks to Australian Paper Maryvale Mill for providing the Bleached Eucalypt Kraft pulp and especially to Opal Ltd. for the continuous support of the project. We thank Rafik Faltas and Thilina Gunawardhana for their assistance and for allowing access to the Opal R&D Centre.
Publisher Copyright:
© 2022, The Author(s).
PY - 2022/3
Y1 - 2022/3
N2 - Glucanases provide an excellent option for pre-treatment of cellulose and improvement in properties of microfibrillated cellulose. In our research, we have used two different enzyme formulations, a purified β-1,4-endoglucanase, and an β-1,4-exoglucanase rich mixture and the focus of the study is on effect of high protein applications on PFI refined-microfibrillated cellulose (2,500 rev). At high protein applications, cellulose fibers showed improved fibrillation after endoglucanase treatment with 20.4% improvement over PFI-only refined fibers while the exoglucanase mix showed 6.8% improvement. At low protein applications, the purified endoglucanase treatment resulted in improved tensile, compression, Scott ply bond, extensional stiffness of microfibrillated cellulose compared with the exoglucanase mix or untreated refined fibers. Scott Ply Bond measurements are an indicator of hydroxyl groups on the fiber surface whose value increased with enzyme pre-treatment. Mechanical properties changed with enzyme treatment and suggest the bonding between fibers increases with endoglucanase treatment providing improved surface functionalization.
AB - Glucanases provide an excellent option for pre-treatment of cellulose and improvement in properties of microfibrillated cellulose. In our research, we have used two different enzyme formulations, a purified β-1,4-endoglucanase, and an β-1,4-exoglucanase rich mixture and the focus of the study is on effect of high protein applications on PFI refined-microfibrillated cellulose (2,500 rev). At high protein applications, cellulose fibers showed improved fibrillation after endoglucanase treatment with 20.4% improvement over PFI-only refined fibers while the exoglucanase mix showed 6.8% improvement. At low protein applications, the purified endoglucanase treatment resulted in improved tensile, compression, Scott ply bond, extensional stiffness of microfibrillated cellulose compared with the exoglucanase mix or untreated refined fibers. Scott Ply Bond measurements are an indicator of hydroxyl groups on the fiber surface whose value increased with enzyme pre-treatment. Mechanical properties changed with enzyme treatment and suggest the bonding between fibers increases with endoglucanase treatment providing improved surface functionalization.
KW - Aspect ratio
KW - Endoglucanase
KW - Exoglucanase
KW - Handsheet properties
KW - Microfibrillated cellulose
KW - PFI refining
UR - http://www.scopus.com/inward/record.url?scp=85124397534&partnerID=8YFLogxK
U2 - 10.1007/s10570-022-04451-7
DO - 10.1007/s10570-022-04451-7
M3 - Article
AN - SCOPUS:85124397534
SN - 0969-0239
VL - 29
SP - 2323
EP - 2332
JO - Cellulose
JF - Cellulose
ER -