The success of pathogenic mycobacterial species is owing in part to their ability to parasitize the generally inhospitable phagosomal environment of host macrophages, utilizing a variety of strategies to avoid their antimycobacterial capabilities and thereby enabling their survival. A recently identified gene target in Mycobacterium smegmatis, highly conserved within Mycobacterium spp. and denoted MSMEG_5817, has been found to be important for bacterial survival within host macrophages. To gain insight into its function, the crystal structure of MSMEG_5817 has been solved to 2.40 A resolution. The structure reveals a high level of structural homology to the sterol carrier protein (SCP) family, suggesting a potential role of MSMEG_5817 in the binding and transportation of biologically relevant lipids required for bacterial survival. The lipid-binding capacity of MSMEG_5817 was confirmed by ELISA, revealing binding to a number of phospholipids with varying binding specificities compared with Homo sapiens SCP. A potential lipid-binding site was probed by alanine-scanning mutagenesis, revealing structurally relevant residues and a binding mechanism potentially differing from that of the SCPs.
|Pages (from-to)||2264 - 2276|
|Number of pages||13|
|Journal||Acta Crystallographica Section D: Biological Crystallography|
|Publication status||Published - 2014|
Shahine, A. E., Littler, D., Brammananth, R., Chan, P. Y., Crellin, P. K., Coppel, R. L., Rossjohn, J., & Beddoe, T. C. (2014). A structural and functional investigation of a novel protein from Mycobacterium smegmatis implicated in mycobacterial macrophage survivability. Acta Crystallographica Section D: Biological Crystallography, 70(9), 2264 - 2276. https://doi.org/10.1107/S139900471401092X