A stable leukocyte-derived factor inhibits platelet aggregation

James J. Liu, Bing Xie, Ian L. Smith, Colin I. Johnston, Brian F. Buxton

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The effect of human polymorphonuclear leukocyte (PMN)-derived supernatants on platelet aggregation was studied. The results showed that the supernatants produced a concentration-dependent inhibition of the platelet aggregation in response to thrombin. The effect was mainly on the second phase of the aggregation, suggesting that the secretory mechanisms of the platelets are disturbed. The activity of the supernatants was not reduced after treatment with heat (95°C for 30 minutes), extreme pH (pH 12 or pH 2) or protease. In conclusion, human PMNs release a stable non-protein factor that inhibits platelet aggregation.

Original languageEnglish
Pages (from-to)878-882
Number of pages5
JournalBiochemical and Biophysical Research Communications
Issue number2
Publication statusPublished - 15 Jun 1994
Externally publishedYes

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