A spindle assembly checkpoint protein functions in prophase I arrest and prometaphase progression

Hayden Homer, Liming Gui, John Graham Carroll

Research output: Contribution to journalArticleResearchpeer-review

105 Citations (Scopus)

Abstract

Two critical stages of mammalian oocyte regulation are prophase I arrest, which is important for sustaining the oocyte pool, and the progression through meiosis I (MI) to produce fertilizable eggs. We have found that the spindle assembly checkpoint protein BubR1 regulates both stages in mouse oocytes. We show that oocytes depleted of BubR1 cannot sustain prophase I arrest and readily undergo germinal vesicle breakdown, a marker for reentry into MI. BubR1-depleted oocytes then arrest before completing MI, marked by failure of polar body extrusion. Both meiotic defects in BubR1-depleted oocytes are due to reduced activity of the master regulator known as the anaphase-promoting complex (APC), brought about through diminished levels of the APC coactivator Cdh1.
Original languageEnglish
Pages (from-to)991 - 994
Number of pages4
JournalScience
Volume326
Issue number5955
DOIs
Publication statusPublished - 2009
Externally publishedYes

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