A somatic proteoglycan controls Notch-directed germ cell fate

Sandeep Gopal; Aqilah Amran; Andre Elton; Leelee Ng; Roger Pocock

doi:10.1038/s41467-021-27039-4

A somatic proteoglycan controls Notch-directed germ cell fate

Sandeep Gopal, Aqilah Amran, Andre Elton, Leelee Ng, Roger Pocock

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11 Citations (Scopus)

Abstract

Communication between the soma and germline optimizes germ cell fate programs. Notch receptors are key determinants of germ cell fate but how somatic signals direct Notch-dependent germ cell behavior is undefined. Here we demonstrate that SDN-1 (syndecan-1), a somatic transmembrane proteoglycan, controls expression of the GLP-1 (germline proliferation-1) Notch receptor in the Caenorhabditis elegans germline. We find that SDN-1 control of a somatic TRP calcium channel governs calcium-dependent binding of an AP-2 transcription factor (APTF-2) to the glp-1 promoter. Hence, SDN-1 signaling promotes GLP-1 expression and mitotic germ cell fate. Together, these data reveal SDN-1 as a putative communication nexus between the germline and its somatic environment to control germ cell fate decisions.

Original language	English
Article number	6708
Number of pages	13
Journal	Nature Communications
Volume	12
Issue number	1
DOIs	https://doi.org/10.1038/s41467-021-27039-4
Publication status	Published - 18 Nov 2021

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title = "A somatic proteoglycan controls Notch-directed germ cell fate",

abstract = "Communication between the soma and germline optimizes germ cell fate programs. Notch receptors are key determinants of germ cell fate but how somatic signals direct Notch-dependent germ cell behavior is undefined. Here we demonstrate that SDN-1 (syndecan-1), a somatic transmembrane proteoglycan, controls expression of the GLP-1 (germline proliferation-1) Notch receptor in the Caenorhabditis elegans germline. We find that SDN-1 control of a somatic TRP calcium channel governs calcium-dependent binding of an AP-2 transcription factor (APTF-2) to the glp-1 promoter. Hence, SDN-1 signaling promotes GLP-1 expression and mitotic germ cell fate. Together, these data reveal SDN-1 as a putative communication nexus between the germline and its somatic environment to control germ cell fate decisions.",

author = "Sandeep Gopal and Aqilah Amran and Andre Elton and Leelee Ng and Roger Pocock",

note = "Funding Information: Some strains were provided by the Caenorhabditis Genetics Center (University of Minnesota), which is funded by the NIH Office of Research Infrastructure Programs (P40 OD010440). We extend our thanks Guangshou Ou and Zhiwen Zu for their expertise and advice in genome-editing to generate CRISPR/Cas9 tagged APTF-2::GFP. We also appreciate Judith Kimble and Sarah Crittenden for making the GLP-1::V5 CRISPR/Cas9 strain available prior to publication, for the kind gift of sdn-1 Minimos rescue constructs by Hannes Bulow and Dayse S. da Cunha, and Monash MicroImaging for their support. We also thank Kyuhyung Kim for the trp-2p::mCherry transgene. This work was supported by the following grants: NHMRC (GNT1105374 and GNT1137645 to R.P.; GNT1161439 to S.G.), ARC (DP200103293 to R.P. and DE190100174 to S.G.), and veski Innovation Fellowship (VIF23 to R.P.). Publisher Copyright: {\textcopyright} 2021, The Author(s).",

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AU - Ng, Leelee

AU - Pocock, Roger

N1 - Funding Information: Some strains were provided by the Caenorhabditis Genetics Center (University of Minnesota), which is funded by the NIH Office of Research Infrastructure Programs (P40 OD010440). We extend our thanks Guangshou Ou and Zhiwen Zu for their expertise and advice in genome-editing to generate CRISPR/Cas9 tagged APTF-2::GFP. We also appreciate Judith Kimble and Sarah Crittenden for making the GLP-1::V5 CRISPR/Cas9 strain available prior to publication, for the kind gift of sdn-1 Minimos rescue constructs by Hannes Bulow and Dayse S. da Cunha, and Monash MicroImaging for their support. We also thank Kyuhyung Kim for the trp-2p::mCherry transgene. This work was supported by the following grants: NHMRC (GNT1105374 and GNT1137645 to R.P.; GNT1161439 to S.G.), ARC (DP200103293 to R.P. and DE190100174 to S.G.), and veski Innovation Fellowship (VIF23 to R.P.). Publisher Copyright: © 2021, The Author(s).

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N2 - Communication between the soma and germline optimizes germ cell fate programs. Notch receptors are key determinants of germ cell fate but how somatic signals direct Notch-dependent germ cell behavior is undefined. Here we demonstrate that SDN-1 (syndecan-1), a somatic transmembrane proteoglycan, controls expression of the GLP-1 (germline proliferation-1) Notch receptor in the Caenorhabditis elegans germline. We find that SDN-1 control of a somatic TRP calcium channel governs calcium-dependent binding of an AP-2 transcription factor (APTF-2) to the glp-1 promoter. Hence, SDN-1 signaling promotes GLP-1 expression and mitotic germ cell fate. Together, these data reveal SDN-1 as a putative communication nexus between the germline and its somatic environment to control germ cell fate decisions.

AB - Communication between the soma and germline optimizes germ cell fate programs. Notch receptors are key determinants of germ cell fate but how somatic signals direct Notch-dependent germ cell behavior is undefined. Here we demonstrate that SDN-1 (syndecan-1), a somatic transmembrane proteoglycan, controls expression of the GLP-1 (germline proliferation-1) Notch receptor in the Caenorhabditis elegans germline. We find that SDN-1 control of a somatic TRP calcium channel governs calcium-dependent binding of an AP-2 transcription factor (APTF-2) to the glp-1 promoter. Hence, SDN-1 signaling promotes GLP-1 expression and mitotic germ cell fate. Together, these data reveal SDN-1 as a putative communication nexus between the germline and its somatic environment to control germ cell fate decisions.

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A somatic proteoglycan controls Notch-directed germ cell fate

Abstract

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A somatic proteoglycan controls Notch-directed germ cell fate

Abstract

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Investigating a novel factor impacting stem cell development

CALCIUM-MEDIATED REGULATION OF STEM CELL DEVELOPMENT

Role of Transient Ca2+ in Transcriptional Wiring of Stem Cells

Cite this