TY - JOUR
T1 - A soft and ultrasensitive force sensing diaphragm for probing cardiac organoids instantaneously and wirelessly
AU - Lyu, Quanxia
AU - Gong, Shu
AU - Lees, Jarmon G.
AU - Yin, Jialiang
AU - Yap, Lim Wei
AU - Kong, Anne M.
AU - Shi, Qianqian
AU - Fu, Runfang
AU - Zhu, Qiang
AU - Dyer, Ash
AU - Dyson, Jennifer M.
AU - Lim, Shiang Y.
AU - Cheng, Wenlong
N1 - Funding Information:
This research was financially supported under Discovery Projects funding scheme (DP200100624), NHMRC Investigator Grant Leadership Fellow (APP2010154) and Jack Brockhoff foundation (JBF Grant number 4659-2019). This work was performed in part at the Melbourne Centre for Nanofabrication (MCN) in the Victorian Node of the Australian National Fabrication Facility (ANFF). This work was performed with support from the St Vincent’s Hospital (Melbourne) Research Endowment Fund and Stafford Fox Medical Research Foundation. The O’Brien Institute Department of St Vincent’s Institute of Medical Research receives Operational Infrastructure Support from the Victorian State Government’s Department of Innovation, Industry and Regional Development.
Funding Information:
This research was financially supported under Discovery Projects funding scheme (DP200100624), NHMRC Investigator Grant Leadership Fellow (APP2010154) and Jack Brockhoff foundation (JBF Grant number 4659-2019). This work was performed in part at the Melbourne Centre for Nanofabrication (MCN) in the Victorian Node of the Australian National Fabrication Facility (ANFF). This work was performed with support from the St Vincent’s Hospital (Melbourne) Research Endowment Fund and Stafford Fox Medical Research Foundation. The O’Brien Institute Department of St Vincent’s Institute of Medical Research receives Operational Infrastructure Support from the Victorian State Government’s Department of Innovation, Industry and Regional Development.
Publisher Copyright:
© 2022, The Author(s).
PY - 2022/11/25
Y1 - 2022/11/25
N2 - Time-lapse mechanical properties of stem cell derived cardiac organoids are important biological cues for understanding contraction dynamics of human heart tissues, cardiovascular functions and diseases. However, it remains difficult to directly, instantaneously and accurately characterize such mechanical properties in real-time and in situ because cardiac organoids are topologically complex, three-dimensional soft tissues suspended in biological media, which creates a mismatch in mechanics and topology with state-of-the-art force sensors that are typically rigid, planar and bulky. Here, we present a soft resistive force-sensing diaphragm based on ultrasensitive resistive nanocracked platinum film, which can be integrated into an all-soft culture well via an oxygen plasma-enabled bonding process. We show that a reliable organoid-diaphragm contact can be established by an ‘Atomic Force Microscope-like’ engaging process. This allows for instantaneous detection of the organoids’ minute contractile forces and beating patterns during electrical stimulation, resuscitation, drug dosing, tissue culture, and disease modelling.
AB - Time-lapse mechanical properties of stem cell derived cardiac organoids are important biological cues for understanding contraction dynamics of human heart tissues, cardiovascular functions and diseases. However, it remains difficult to directly, instantaneously and accurately characterize such mechanical properties in real-time and in situ because cardiac organoids are topologically complex, three-dimensional soft tissues suspended in biological media, which creates a mismatch in mechanics and topology with state-of-the-art force sensors that are typically rigid, planar and bulky. Here, we present a soft resistive force-sensing diaphragm based on ultrasensitive resistive nanocracked platinum film, which can be integrated into an all-soft culture well via an oxygen plasma-enabled bonding process. We show that a reliable organoid-diaphragm contact can be established by an ‘Atomic Force Microscope-like’ engaging process. This allows for instantaneous detection of the organoids’ minute contractile forces and beating patterns during electrical stimulation, resuscitation, drug dosing, tissue culture, and disease modelling.
UR - http://www.scopus.com/inward/record.url?scp=85142476791&partnerID=8YFLogxK
U2 - 10.1038/s41467-022-34860-y
DO - 10.1038/s41467-022-34860-y
M3 - Article
C2 - 36433978
AN - SCOPUS:85142476791
SN - 2041-1723
VL - 13
JO - Nature Communications
JF - Nature Communications
IS - 1
M1 - 7259
ER -
