A single-molecule study on the structural damage of ultraviolet radiated DNA

Kathryn Schallhorn; Mickey Kim; Chun Ke Pu

doi:10.3390/ijms9040662

A single-molecule study on the structural damage of ultraviolet radiated DNA

Kathryn Schallhorn, Mickey Kim, Chun Ke Pu

Research output: Contribution to journal › Article › Research › peer-review

4 Citations (Scopus)

Abstract

The structural damage of double-stranded DNA under UV radiation was examined using single-molecule fluorescence microscopy. Compared to undamaged DNA, the diffusion coefficient of λ-DNA was significantly increased with 12 min or 20 min of radiation but remained unchanged for 40 min of exposure possibly due to strand crosslinking. The structural damage of DNA was further examined using transmission electron microscopy which revealed kinks and sharp bends along the DNA backbone.

Original language	English
Pages (from-to)	662-667
Number of pages	6
Journal	International Journal of Molecular Sciences
Volume	9
Issue number	4
DOIs	https://doi.org/10.3390/ijms9040662
Publication status	Published - 1 Apr 2008
Externally published	Yes

Keywords

Diffusion
DNA damage
Flexibility
UV radiation

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10.3390/ijms9040662

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abstract = "The structural damage of double-stranded DNA under UV radiation was examined using single-molecule fluorescence microscopy. Compared to undamaged DNA, the diffusion coefficient of λ-DNA was significantly increased with 12 min or 20 min of radiation but remained unchanged for 40 min of exposure possibly due to strand crosslinking. The structural damage of DNA was further examined using transmission electron microscopy which revealed kinks and sharp bends along the DNA backbone.",

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AB - The structural damage of double-stranded DNA under UV radiation was examined using single-molecule fluorescence microscopy. Compared to undamaged DNA, the diffusion coefficient of λ-DNA was significantly increased with 12 min or 20 min of radiation but remained unchanged for 40 min of exposure possibly due to strand crosslinking. The structural damage of DNA was further examined using transmission electron microscopy which revealed kinks and sharp bends along the DNA backbone.

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KW - DNA damage

KW - Flexibility

KW - UV radiation

UR - https://www.scopus.com/pages/publications/43049102046

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DO - 10.3390/ijms9040662

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A single-molecule study on the structural damage of ultraviolet radiated DNA

Abstract

Keywords

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