A single-molecule study on the structural damage of ultraviolet radiated DNA

Kathryn Schallhorn, Mickey Kim, Chun Ke Pu

Research output: Contribution to journalArticleResearchpeer-review

2 Citations (Scopus)

Abstract

The structural damage of double-stranded DNA under UV radiation was examined using single-molecule fluorescence microscopy. Compared to undamaged DNA, the diffusion coefficient of λ-DNA was significantly increased with 12 min or 20 min of radiation but remained unchanged for 40 min of exposure possibly due to strand crosslinking. The structural damage of DNA was further examined using transmission electron microscopy which revealed kinks and sharp bends along the DNA backbone.

Original languageEnglish
Pages (from-to)662-667
Number of pages6
JournalInternational Journal of Molecular Sciences
Volume9
Issue number4
DOIs
Publication statusPublished - 1 Apr 2008
Externally publishedYes

Keywords

  • Diffusion
  • DNA damage
  • Flexibility
  • UV radiation

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