A simple protocol to obtain highly pure Wolbachia endosymbiont DNA for genome sequencing

Inaki Iturbe-Ormaetxe, Megan Woolfit, Edwige Rances, Anne Duplouy, Scott Leslie O'Neill

Research output: Contribution to journalArticleOtherpeer-review

20 Citations (Scopus)

Abstract

Most genome sequencing projects using intracellular bacteria face difficulties in obtaining sufficient bacterial DNA free of host contamination. We have developed a simple and rapid protocol to isolate endosymbiont DNA virtually free from fly and mosquito host DNA. We purified DNA from six Wolbachia strains in preparation for genome sequencing using this method, and achieved up to 97 pure Wolbachia sequence, even after using frozen insects. This is a significant improvement for future Wolbachia and other endosymbiont genome projects.
Original languageEnglish
Pages (from-to)134 - 136
Number of pages3
JournalJournal of Microbiological Methods
Volume84
Issue number1
DOIs
Publication statusPublished - 2011
Externally publishedYes

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