A simple method for the assay of colistin in human plasma, using pre-column derivatization with 9-fluorenylmethyl chloroformate in solid-phase extraction cartridges and reversed-phase high-performance liquid chromatography

Jian Li, Robert W. Milne, Roger L. Nation, John D. Turnidge, Kingsley Coulthard, David W. Johnson

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A simple, selective and sensitive high-performance liquid chromatographic (HPLC) method is described for the determination of colistin in human plasma. Derivatization with 9-fluorenylmethyl chloroformate was performed in the same solid-phase extraction C18 cartridge used for sample pre-treatment, followed by reversed-phase HPLC with fluorimetric detection. Quantification was achieved using the ratio of the summed peak areas of colistin A and B derivatives to that of the derivative of netilmicin (internal standard). Linear calibration curves were obtained within the concentrations of colistin sulfate from 0.10 to 4.0 mg/l in plasma. Accuracy was within 10% and reproducibility (RSD) was less than 10%.

Original languageEnglish
Pages (from-to)167-175
Number of pages9
JournalJournal of Chromatography B: Biomedical Sciences and Applications
Issue number2
Publication statusPublished - 25 Sep 2001


  • 9-Flourenylmethyl chloroformate
  • Colistin
  • Derivatization, LC

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