A simple and economical DRB1 typing procedure combining group‐specific amplification, DNA heteroduplex and enzyme restriction analysis

Mauro D'Amato, Rosa Sorrentino

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12 Citations (Scopus)


Abstract: A new procedure for HLA‐DRB1 typing is proposed. The method combines six group‐specific amplifications with heteroduplex analysis and, in some cases, enzyme restriction analysis. This technique, which is as discriminative as oligotyping, is simple, economical and does not require probes. These characteristics make this approach a valid alternative to other HLA genomic typing procedures, especially in those cases such as donor‐recipient pairs matching where a small number of samples has to be managed at once.

Original languageEnglish
Pages (from-to)295-301
Number of pages7
JournalTissue Antigens
Issue number5
Publication statusPublished - 1 Jan 1994
Externally publishedYes


  • group‐specific PCR
  • heteroduplex analysis
  • HLA‐DR

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