A serpin in the cellulosome of the anaerobic fungus Piromyces sp. strain E2

Peter JM Steenbakkers, James Alexander Irving, Harry R Harhangi, Willem JC Swinkels, Anna Akhmanova, Rembrandt Dijkerman, Mike SM Jetten, Chris Van Der Drift, James Whisstock, Huub JM Op Den Camp

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Abstract

A gene encoding a novel component of the cellulolytic complex (cellulosome) of the anaerobic fungus Piromyces sp. strain E2 was identified. The encoded 538 amino acid protein, named celpin, consists of a signal peptide, a positively charged domain of unknown function followed by two fungal dockerins, typical for components of the extracellular fungal cellulosome. The C-terminal end consists of a 380 amino acid serine proteinase inhibitor (or serpin) domain homologue, sharing 30 identity and 50 similarity to vertebrate and bacterial serpins. Detailed protein sequence analysis of the serpin domain revealed that it contained all features of a functional serpin. It possesses the conserved amino acids present in more than 70 of known serpins, and it contained the consensus of inhibiting serpins. Because of the confined space of the fungal cellulosome inside plant tissue and the auto-proteolysis of plant material in the rumen, the fungal serpin is presumably involved in protection of the cellulosome against plant proteinases. The celpin protein of Piromyces sp. strain E2 is the first non-structural, non-hydrolytic fungal cellulosome component. Furthermore, the celpin protein of Piromyces sp. strain E2 is the first representative of a serine proteinase inhibitor of the fungal kingdom.
Original languageEnglish
Pages (from-to)999 - 1006
Number of pages8
JournalMycological Research
Volume112
Issue number8
Publication statusPublished - 2008

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