A serological assay to detect SARS-CoV-2 seroconversion in humans

Fatima Amanat, Daniel Stadlbauer, Shirin Strohmeier, Thi H.O. Nguyen, Veronika Chromikova, Meagan McMahon, Kaijun Jiang, Guha Asthagiri Arunkumar, Denise Jurczyszak, Jose Polanco, Maria Bermudez-Gonzalez, Giulio Kleiner, Teresa Aydillo, Lisa Miorin, Daniel S. Fierer, Luz Amarilis Lugo, Erna Milunka Kojic, Jonathan Stoever, Sean T.H. Liu, Charlotte Cunningham-RundlesPhilip L. Felgner, Thomas Moran, Adolfo García-Sastre, Daniel Caplivski, Allen C. Cheng, Katherine Kedzierska, Olli Vapalahti, Jussi M. Hepojoki, Viviana Simon, Florian Krammer

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Here, we describe a serological enzyme-linked immunosorbent assay for the screening and identification of human SARS-CoV-2 seroconverters. This assay does not require the handling of infectious virus, can be adjusted to detect different antibody types in serum and plasma and is amenable to scaling. Serological assays are of critical importance to help define previous exposure to SARS-CoV-2 in populations, identify highly reactive human donors for convalescent plasma therapy and investigate correlates of protection.

Original languageEnglish
Pages (from-to)1033-1036
Number of pages4
JournalNature Medicine
Issue number7
Publication statusPublished - Jul 2020

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