A sample preparation protocol for 1H nuclear magnetic resonance studies of water-soluble metabolites in blood and urine

John Robert Sheedy, Peter R. Ebeling, Paul Raymond Gooley, Malcolm J McConville

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27 Citations (Scopus)

Abstract

We describe a general protocol for preparing protein-containing biofluids for 1H nuclear magnetic resonance (NMR) metabolomic studies. In this protocol, untreated samples are diluted in deuterated solvents to precipitate proteins and recover metabolites quantitated relative to standard reference compounds such as 3-trimethylsilylpropionic acid (TSP) and 2,2-dimethyl-2-silapentane-5-sulfonic acid (DSS). The efficacy of this protocol was tested using a bovine serum albumin/metabolite mix and human serum samples. This sample preparation method can be readily applied to any protein-containing biofluid for 1H NMR studies.

Original languageEnglish
Pages (from-to)263-265
Number of pages3
JournalAnalytical Biochemistry
Volume398
Issue number2
DOIs
Publication statusPublished - Mar 2010
Externally publishedYes

Keywords

  • Biofluids
  • Metabolites
  • NMR

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