A ratiometric fluorescent sensor for the mitochondrial copper pool

Clara Shen; Jacek L. Kolanowski; Carmen M.N. Tran; Amandeep Kaur; Mia C. Akerfeldt; Matthew S. Rahme; Trevor W. Hambley; Elizabeth J. New

doi:10.1039/c6mt00083e

A ratiometric fluorescent sensor for the mitochondrial copper pool

Clara Shen, Jacek L. Kolanowski, Carmen M.N. Tran, Amandeep Kaur, Mia C. Akerfeldt, Matthew S. Rahme, Trevor W. Hambley, Elizabeth J. New

Research output: Contribution to journal › Article › Research › peer-review

35 Citations (Scopus)

Abstract

Copper plays a key role in the modulation of cellular function, defence, and growth. Here we present InCCu1, a ratiometric fluorescent sensor for mitochondrial copper, which changes from red to blue emission in the presence of Cu(i). Employing this probe in microscopy and flow cytometry, we show that cisplatin-treated cells have an impaired ability to accumulate copper in the mitochondria.

Original language	English
Pages (from-to)	915-919
Number of pages	5
Journal	Metallomics
Volume	8
Issue number	9
DOIs	https://doi.org/10.1039/c6mt00083e
Publication status	Published - Sept 2016
Externally published	Yes

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title = "A ratiometric fluorescent sensor for the mitochondrial copper pool",

abstract = "Copper plays a key role in the modulation of cellular function, defence, and growth. Here we present InCCu1, a ratiometric fluorescent sensor for mitochondrial copper, which changes from red to blue emission in the presence of Cu(i). Employing this probe in microscopy and flow cytometry, we show that cisplatin-treated cells have an impaired ability to accumulate copper in the mitochondria.",

author = "Clara Shen and Kolanowski, {Jacek L.} and Tran, {Carmen M.N.} and Amandeep Kaur and Akerfeldt, {Mia C.} and Rahme, {Matthew S.} and Hambley, {Trevor W.} and New, {Elizabeth J.}",

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AU - Tran, Carmen M.N.

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AU - Akerfeldt, Mia C.

AU - Rahme, Matthew S.

AU - Hambley, Trevor W.

AU - New, Elizabeth J.

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AB - Copper plays a key role in the modulation of cellular function, defence, and growth. Here we present InCCu1, a ratiometric fluorescent sensor for mitochondrial copper, which changes from red to blue emission in the presence of Cu(i). Employing this probe in microscopy and flow cytometry, we show that cisplatin-treated cells have an impaired ability to accumulate copper in the mitochondria.

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A ratiometric fluorescent sensor for the mitochondrial copper pool

Abstract

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