Abstract
We present a first instance of performing 3D differentiation of a human liver stem cell in a pumpfree microfluidic platform. Pump-free perfusion culture at constant flow rate was achieved by using 3D printed supports to control the hydrostatic head difference between a pair of horizontally orientated media reservoirs connected to a microfluidic 3D cell culture device. We demonstrate that on-chip differentiation of HepaRG cells, a human bipotent liver stem cell, could efficiently generate functional hepatocytes and biliary cells, for up to 19 days.
| Original language | English |
|---|---|
| Title of host publication | 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2016) |
| Editors | Nicole Pamme, Jens Ducrée |
| Place of Publication | United States |
| Publisher | Chemical and Biological Microsystems Society |
| Pages | 395-396 |
| Number of pages | 2 |
| ISBN (Electronic) | 9780979806490 |
| ISBN (Print) | 9781510834163 |
| Publication status | Published - 2016 |
| Externally published | Yes |
| Event | International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2016) - Convention Center Dublin, Dublin, Ireland Duration: 9 Oct 2016 → 13 Oct 2016 Conference number: 20th |
Conference
| Conference | International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2016) |
|---|---|
| Abbreviated title | MicroTAS 2016 |
| Country/Territory | Ireland |
| City | Dublin |
| Period | 9/10/16 → 13/10/16 |
| Other | The Twentieth International Conference on Miniaturized Systems for Chemistry and Life Sciences (µTAS 2016) will be held at the Convention Center Dublin in Dublin, IRELAND from 9 - 13 October 2016. |
Keywords
- HepaRG differentiation
- Liver-in-chip
- Microfluidics
- Pump-free