A protocol for the preparation of cryoprecipitate and cryodepleted plasma

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Abstract

Cryoprecipitate is a concentrate of high-molecular-weight plasma proteins that precipitate when frozen plasma is slowly thawed at 1a??6A?C. The concentrate contains factor VIII (antihemophilic factor), von Willebrand factor (vWF), fibrinogen, factor XIII, fibronectin, and small amounts of other plasma proteins. Clinical-grade preparations of cryoprecipitate are mainly used to treat fibrinogen deficiency caused by acute bleeding or functional abnormalities of the fibrinogen protein. In the past, cryoprecipitate was used to treat von Willebrand disease and hemophilia A (factor VIII deficiency), but the availability of more highly purified coagulation factor concentrates or recombinant protein preparations has superseded the use of cryoprecipitate for these coagulopathies. Cryodepleted plasma (a??cryosupernatanta??) is the plasma supernatant that remains following removal of the cryoprecipitate from frozen-thawed plasma. It contains all the other plasma proteins and clotting factors present in plasma that remain soluble during cold-temperature thawing of the plasma.
Original languageEnglish
Title of host publicationSerum/Plasma Proteomics: Methods and Protocols
EditorsRichard J Simpson, David W Greening
Place of PublicationUK
PublisherHumana Press
Pages259 - 265
Number of pages7
Volume728
ISBN (Print)9781617790676
DOIs
Publication statusPublished - 2011

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