Abstract
| Original language | English |
|---|---|
| Title of host publication | Serum/Plasma Proteomics: Methods and Protocols |
| Editors | Richard J Simpson, David W Greening |
| Place of Publication | UK |
| Publisher | Humana Press |
| Pages | 259 - 265 |
| Number of pages | 7 |
| Volume | 728 |
| ISBN (Print) | 9781617790676 |
| DOIs | |
| Publication status | Published - 2011 |
Cite this
}
A protocol for the preparation of cryoprecipitate and cryodepleted plasma. / Sparrow, Rosemary; Greening, David W; Simpson, Richard J.
Serum/Plasma Proteomics: Methods and Protocols. ed. / Richard J Simpson; David W Greening. Vol. 728 UK : Humana Press, 2011. p. 259 - 265.Research output: Chapter in Book/Report/Conference proceeding › Chapter (Book) › Other
TY - CHAP
T1 - A protocol for the preparation of cryoprecipitate and cryodepleted plasma
AU - Sparrow, Rosemary
AU - Greening, David W
AU - Simpson, Richard J
PY - 2011
Y1 - 2011
N2 - Cryoprecipitate is a concentrate of high-molecular-weight plasma proteins that precipitate when frozen plasma is slowly thawed at 1a??6A?C. The concentrate contains factor VIII (antihemophilic factor), von Willebrand factor (vWF), fibrinogen, factor XIII, fibronectin, and small amounts of other plasma proteins. Clinical-grade preparations of cryoprecipitate are mainly used to treat fibrinogen deficiency caused by acute bleeding or functional abnormalities of the fibrinogen protein. In the past, cryoprecipitate was used to treat von Willebrand disease and hemophilia A (factor VIII deficiency), but the availability of more highly purified coagulation factor concentrates or recombinant protein preparations has superseded the use of cryoprecipitate for these coagulopathies. Cryodepleted plasma (a??cryosupernatanta??) is the plasma supernatant that remains following removal of the cryoprecipitate from frozen-thawed plasma. It contains all the other plasma proteins and clotting factors present in plasma that remain soluble during cold-temperature thawing of the plasma.
AB - Cryoprecipitate is a concentrate of high-molecular-weight plasma proteins that precipitate when frozen plasma is slowly thawed at 1a??6A?C. The concentrate contains factor VIII (antihemophilic factor), von Willebrand factor (vWF), fibrinogen, factor XIII, fibronectin, and small amounts of other plasma proteins. Clinical-grade preparations of cryoprecipitate are mainly used to treat fibrinogen deficiency caused by acute bleeding or functional abnormalities of the fibrinogen protein. In the past, cryoprecipitate was used to treat von Willebrand disease and hemophilia A (factor VIII deficiency), but the availability of more highly purified coagulation factor concentrates or recombinant protein preparations has superseded the use of cryoprecipitate for these coagulopathies. Cryodepleted plasma (a??cryosupernatanta??) is the plasma supernatant that remains following removal of the cryoprecipitate from frozen-thawed plasma. It contains all the other plasma proteins and clotting factors present in plasma that remain soluble during cold-temperature thawing of the plasma.
UR - http://www.springer.com/new+%26+forthcoming+titles+(default)/book/978-1-61779-067-6
U2 - 10.1007/978-1-61779-068-3_17
DO - 10.1007/978-1-61779-068-3_17
M3 - Chapter (Book)
SN - 9781617790676
VL - 728
SP - 259
EP - 265
BT - Serum/Plasma Proteomics: Methods and Protocols
A2 - Simpson, Richard J
A2 - Greening, David W
PB - Humana Press
CY - UK
ER -