A protocol for the preparation of cryoprecipitate and cryo-depleted plasma for proteomic studies

Research output: Chapter in Book/Report/Conference proceedingChapter (Book)Otherpeer-review

Abstract

Cryoprecipitate is a concentrate of high-molecular-weight plasma proteins that precipitate when frozen plasma is slowly thawed at 1–6 °C. The concentrate contains factor VIII (antihemophilic factor), von Willebrand factor (vWF), fibrinogen, factor XIII, fibronectin, and small amounts of other plasma proteins. Clinical grade preparations of cryoprecipitate are mainly used to treat fibrinogen deficiency caused by acute bleeding or functional abnormalities of the fibrinogen protein. In the past, cryoprecipitate was used to treat von Willebrand disease and hemophilia A (factor VIII deficiency), but the availability of more highly purified coagulation factor concentrates or recombinant protein preparations has superseded the use of cryoprecipitate for these coagulopathies. Cryo-depleted plasma (“cryosupernatant”) is the plasma supernatant remaining following removal of the cryoprecipitate from frozen-thawed plasma. It contains all the other plasma proteins and clotting factors present in plasma that remain soluble during cold-temperature thawing of the plasma. This protocol describes the clinical-scale preparation of cryoprecipitate and cryo-depleted plasma for proteomic studies.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
Subtitle of host publicationSerum/Plasma Proteomics
EditorsDavid W. Greening, Richard J. Simpson
PublisherHumana Press
Pages23-30
Number of pages8
Volume1619
Edition2nd
ISBN (Electronic)978-1-4939-7057-5
ISBN (Print)978-1-4939-7056-8
DOIs
Publication statusPublished - 2017

Publication series

NameMethods in Molecular Biology
Volume1619
ISSN (Print)10643745

Keywords

  • Antihemophilic factor
  • Cryo-depleted
  • Cryoprecipitate
  • Cryosupernatant
  • Factor VIII
  • Fibrinogen
  • Plasma
  • Proteomics

Cite this

Sparrow, R. L., Simpson, R. J., & Greening, D. W. (2017). A protocol for the preparation of cryoprecipitate and cryo-depleted plasma for proteomic studies. In D. W. Greening, & R. J. Simpson (Eds.), Methods in Molecular Biology: Serum/Plasma Proteomics (2nd ed., Vol. 1619, pp. 23-30). (Methods in Molecular Biology; Vol. 1619). Humana Press. https://doi.org/10.1007/978-1-4939-7057-5_2
Sparrow, Rosemary L. ; Simpson, Richard J. ; Greening, David W. / A protocol for the preparation of cryoprecipitate and cryo-depleted plasma for proteomic studies. Methods in Molecular Biology: Serum/Plasma Proteomics. editor / David W. Greening ; Richard J. Simpson. Vol. 1619 2nd. ed. Humana Press, 2017. pp. 23-30 (Methods in Molecular Biology).
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Sparrow, RL, Simpson, RJ & Greening, DW 2017, A protocol for the preparation of cryoprecipitate and cryo-depleted plasma for proteomic studies. in DW Greening & RJ Simpson (eds), Methods in Molecular Biology: Serum/Plasma Proteomics. 2nd edn, vol. 1619, Methods in Molecular Biology, vol. 1619, Humana Press, pp. 23-30. https://doi.org/10.1007/978-1-4939-7057-5_2

A protocol for the preparation of cryoprecipitate and cryo-depleted plasma for proteomic studies. / Sparrow, Rosemary L.; Simpson, Richard J.; Greening, David W.

Methods in Molecular Biology: Serum/Plasma Proteomics. ed. / David W. Greening; Richard J. Simpson. Vol. 1619 2nd. ed. Humana Press, 2017. p. 23-30 (Methods in Molecular Biology; Vol. 1619).

Research output: Chapter in Book/Report/Conference proceedingChapter (Book)Otherpeer-review

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Sparrow RL, Simpson RJ, Greening DW. A protocol for the preparation of cryoprecipitate and cryo-depleted plasma for proteomic studies. In Greening DW, Simpson RJ, editors, Methods in Molecular Biology: Serum/Plasma Proteomics. 2nd ed. Vol. 1619. Humana Press. 2017. p. 23-30. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-4939-7057-5_2