A protocol for the preparation of cryoprecipitate and cryo-depleted plasma for proteomic studies

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Abstract

Cryoprecipitate is a concentrate of high-molecular-weight plasma proteins that precipitate when frozen plasma is slowly thawed at 1–6 °C. The concentrate contains factor VIII (antihemophilic factor), von Willebrand factor (vWF), fibrinogen, factor XIII, fibronectin, and small amounts of other plasma proteins. Clinical grade preparations of cryoprecipitate are mainly used to treat fibrinogen deficiency caused by acute bleeding or functional abnormalities of the fibrinogen protein. In the past, cryoprecipitate was used to treat von Willebrand disease and hemophilia A (factor VIII deficiency), but the availability of more highly purified coagulation factor concentrates or recombinant protein preparations has superseded the use of cryoprecipitate for these coagulopathies. Cryo-depleted plasma (“cryosupernatant”) is the plasma supernatant remaining following removal of the cryoprecipitate from frozen-thawed plasma. It contains all the other plasma proteins and clotting factors present in plasma that remain soluble during cold-temperature thawing of the plasma. This protocol describes the clinical-scale preparation of cryoprecipitate and cryo-depleted plasma for proteomic studies.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
Subtitle of host publicationSerum/Plasma Proteomics
EditorsDavid W. Greening, Richard J. Simpson
PublisherHumana Press
Pages23-30
Number of pages8
Volume1619
Edition2nd
ISBN (Electronic)978-1-4939-7057-5
ISBN (Print)978-1-4939-7056-8
DOIs
Publication statusPublished - 2017

Publication series

NameMethods in Molecular Biology
Volume1619
ISSN (Print)10643745

Keywords

  • Antihemophilic factor
  • Cryo-depleted
  • Cryoprecipitate
  • Cryosupernatant
  • Factor VIII
  • Fibrinogen
  • Plasma
  • Proteomics

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