A promoter that drives transgene expression in cerebellar Purkinje and retinal bipolar neurons

John Oberdick, Richard J. Smeyne, Jeff R. Mann, Saul Zackson, James I. Morgan

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236 Citations (Scopus)

Abstract

A genomic done encoding the Purkinje cell-specific L7 protein has been isolated and utilized to drive the expression of β-galactosidase in mice. Three independent transgenic lines, germ line transformed with an L7-β-galactosidase fusion gene, exhibit β-galactosidase expression in both cerebellar Purkinje cells and retinal bipolar neurons. This distribution is the same as that previously determined for the L7 protein by immunohistochemistry. The transgenic murine lines can be used to obtain populations of marked Purkinje and bipolar neurons. Similar L7 promoter constructs can be used to express other foreign genes specifically in these two classes of neurons.

Original languageEnglish
Pages (from-to)223-226
Number of pages4
JournalScience
Volume248
Issue number4952
Publication statusPublished - 1990
Externally publishedYes

Cite this

Oberdick, J., Smeyne, R. J., Mann, J. R., Zackson, S., & Morgan, J. I. (1990). A promoter that drives transgene expression in cerebellar Purkinje and retinal bipolar neurons. Science, 248(4952), 223-226.