A practical guide to the stereological assessment of glomerular number, size, and cellular composition

Megan R. Sutherland, Danica Vojisavljevic, Mary Jane Black

Research output: Contribution to journalArticleOtherpeer-review

2 Citations (Scopus)

Abstract

The evaluation of a range of measures in the kidneys, such as developmental stage, rate and success, injury, and disease processes, relies on obtaining information on the three-dimensional structure of the renal corpuscles, and in particular the glomerular capillary tufts. To do this in the most accurate, comprehensive, and unbiased manner depends on a knowledge of stereological methods. In this article, we provide a practical guide for researchers on how to quantitate a number of structures in the kidneys, including the estimation of total glomerular number, glomerular capillary length and filtration surface area, and the cellular composition of individual glomeruli. Guidance is also provided on how to apply these methods to kidneys at different sizes and levels of maturity.

Original languageEnglish
Number of pages14
JournalAnatomical Record
DOIs
Publication statusAccepted/In press - 21 Jan 2020

Keywords

  • glomerulus
  • kidney development
  • nephron number
  • podocyte
  • stereology

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