Background Chronic kidney disease (CKD), and its associated cardiovascular events, is one of the major causes of morbidity and recurrent hospitalization in Asian Pacific region. The subtotal nephrectomy (STNx) model has remained the state-of-the-art prototype which closely mimics human CKD and cardiac-renal syndrome. Aim and Methods In this article, we comprehensively outline the procedure and methodology required to develop the rat model 5/6 nephrectomy and the associated procedures involved in assessing cardiac and renal functional outcomes. Results and Conclusion In addition, the expected functional outcomes from our own experience, and those of others, have been described. The STNx model in the rat is an established model of CKD and displays all the functional and structural hallmarks observed in the human condition. Lesser known are the cardiac effects of this model which make it ideal for studying cardiorenal syndrome. Summary at a Glance This manuscript provides a comprehensive guide for performing the subtotal nephrectomy model in rats and includes methodology for assessing renal and cardiac function in this model. It is a useful methods paper that is well suited to the Nephrology journal.