A portable microfluidic-based biophotonic sensor for extracellular H 2O2 measurements

V. Koman, G. Suárez, Ch Santschi, V. J. Cadarso, J. Brugger, N. Von Moos, V. I. Slaveykova, O. J F Martin

Research output: Chapter in Book/Report/Conference proceedingConference PaperResearchpeer-review

Abstract

In this work a portable analytical biosensor for real-time extracellular monitoring of released hydrogen peroxide (H2O2 ) is presented. The biosensor is based on the optical detection of the cytochrome c (cyt c) oxidation state. The setup consists of an integrated microscope combined with a compact spectrometer. The light being absorbed by cyt c is enhanced via multiscattering produced by random aggregates of polystyrene beads in a cross-linked cyt c matrix. Using ink-jet printing technique, the sensing elements, namely cyt c loaded polystyrene aggregates, are fabricated with high reliability in terms of repeatability of size and sensitivity. Additionally, the sensing elements are enclosed in a microfluidic channel assuring a fast and efficient analytes delivery. As an example, the effect of trace concentrations of functionalized cadmium selenide/zinc sulfide (CdSe/ZnS) core shell quantum dots on the green algae Chlamydomonas reinhardtii is investigated, showing extracellular H2O2 release with different production rates over a period of 1 hour. In conclusion, the presented portable biosensor enables the highly sensitive and non-invasive real-time monitoring of the cell metabolism of C. reinhardtii.

Original languageEnglish
Title of host publicationAdvanced Biomedical and Clinical Diagnostic Systems XI
PublisherSPIE
Number of pages7
Volume8572
ISBN (Print)9780819493415
DOIs
Publication statusPublished - 2013
Externally publishedYes
EventAdvanced Biomedical and Clinical Diagnostic Systems XI 2013 - San Francisco, United States of America
Duration: 3 Feb 20135 Feb 2013
https://www.spiedigitallibrary.org/conference-proceedings-of-spie/8572.toc#FrontMatter:Volume8572 (SPIE conference proceedings)

Conference

ConferenceAdvanced Biomedical and Clinical Diagnostic Systems XI 2013
CountryUnited States of America
CitySan Francisco
Period3/02/135/02/13
OtherSPIE Proceedings Volume 8572, Advanced Biomedical and Clinical Diagnostic Systems XI; 857218 (2013)
Internet address

Keywords

  • ink-jet
  • microfluidics
  • oxidative stress
  • portable biosensor
  • quantum dots
  • reactive oxygen species

Cite this

Koman, V., Suárez, G., Santschi, C., Cadarso, V. J., Brugger, J., Von Moos, N., ... Martin, O. J. F. (2013). A portable microfluidic-based biophotonic sensor for extracellular H 2O2 measurements. In Advanced Biomedical and Clinical Diagnostic Systems XI (Vol. 8572). [857218] SPIE. https://doi.org/10.1117/12.2008329
Koman, V. ; Suárez, G. ; Santschi, Ch ; Cadarso, V. J. ; Brugger, J. ; Von Moos, N. ; Slaveykova, V. I. ; Martin, O. J F. / A portable microfluidic-based biophotonic sensor for extracellular H 2O2 measurements. Advanced Biomedical and Clinical Diagnostic Systems XI. Vol. 8572 SPIE, 2013.
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abstract = "In this work a portable analytical biosensor for real-time extracellular monitoring of released hydrogen peroxide (H2O2 ) is presented. The biosensor is based on the optical detection of the cytochrome c (cyt c) oxidation state. The setup consists of an integrated microscope combined with a compact spectrometer. The light being absorbed by cyt c is enhanced via multiscattering produced by random aggregates of polystyrene beads in a cross-linked cyt c matrix. Using ink-jet printing technique, the sensing elements, namely cyt c loaded polystyrene aggregates, are fabricated with high reliability in terms of repeatability of size and sensitivity. Additionally, the sensing elements are enclosed in a microfluidic channel assuring a fast and efficient analytes delivery. As an example, the effect of trace concentrations of functionalized cadmium selenide/zinc sulfide (CdSe/ZnS) core shell quantum dots on the green algae Chlamydomonas reinhardtii is investigated, showing extracellular H2O2 release with different production rates over a period of 1 hour. In conclusion, the presented portable biosensor enables the highly sensitive and non-invasive real-time monitoring of the cell metabolism of C. reinhardtii.",
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author = "V. Koman and G. Su{\'a}rez and Ch Santschi and Cadarso, {V. J.} and J. Brugger and {Von Moos}, N. and Slaveykova, {V. I.} and Martin, {O. J F}",
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Koman, V, Suárez, G, Santschi, C, Cadarso, VJ, Brugger, J, Von Moos, N, Slaveykova, VI & Martin, OJF 2013, A portable microfluidic-based biophotonic sensor for extracellular H 2O2 measurements. in Advanced Biomedical and Clinical Diagnostic Systems XI. vol. 8572, 857218, SPIE, Advanced Biomedical and Clinical Diagnostic Systems XI 2013, San Francisco, United States of America, 3/02/13. https://doi.org/10.1117/12.2008329

A portable microfluidic-based biophotonic sensor for extracellular H 2O2 measurements. / Koman, V.; Suárez, G.; Santschi, Ch; Cadarso, V. J.; Brugger, J.; Von Moos, N.; Slaveykova, V. I.; Martin, O. J F.

Advanced Biomedical and Clinical Diagnostic Systems XI. Vol. 8572 SPIE, 2013. 857218.

Research output: Chapter in Book/Report/Conference proceedingConference PaperResearchpeer-review

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N2 - In this work a portable analytical biosensor for real-time extracellular monitoring of released hydrogen peroxide (H2O2 ) is presented. The biosensor is based on the optical detection of the cytochrome c (cyt c) oxidation state. The setup consists of an integrated microscope combined with a compact spectrometer. The light being absorbed by cyt c is enhanced via multiscattering produced by random aggregates of polystyrene beads in a cross-linked cyt c matrix. Using ink-jet printing technique, the sensing elements, namely cyt c loaded polystyrene aggregates, are fabricated with high reliability in terms of repeatability of size and sensitivity. Additionally, the sensing elements are enclosed in a microfluidic channel assuring a fast and efficient analytes delivery. As an example, the effect of trace concentrations of functionalized cadmium selenide/zinc sulfide (CdSe/ZnS) core shell quantum dots on the green algae Chlamydomonas reinhardtii is investigated, showing extracellular H2O2 release with different production rates over a period of 1 hour. In conclusion, the presented portable biosensor enables the highly sensitive and non-invasive real-time monitoring of the cell metabolism of C. reinhardtii.

AB - In this work a portable analytical biosensor for real-time extracellular monitoring of released hydrogen peroxide (H2O2 ) is presented. The biosensor is based on the optical detection of the cytochrome c (cyt c) oxidation state. The setup consists of an integrated microscope combined with a compact spectrometer. The light being absorbed by cyt c is enhanced via multiscattering produced by random aggregates of polystyrene beads in a cross-linked cyt c matrix. Using ink-jet printing technique, the sensing elements, namely cyt c loaded polystyrene aggregates, are fabricated with high reliability in terms of repeatability of size and sensitivity. Additionally, the sensing elements are enclosed in a microfluidic channel assuring a fast and efficient analytes delivery. As an example, the effect of trace concentrations of functionalized cadmium selenide/zinc sulfide (CdSe/ZnS) core shell quantum dots on the green algae Chlamydomonas reinhardtii is investigated, showing extracellular H2O2 release with different production rates over a period of 1 hour. In conclusion, the presented portable biosensor enables the highly sensitive and non-invasive real-time monitoring of the cell metabolism of C. reinhardtii.

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BT - Advanced Biomedical and Clinical Diagnostic Systems XI

PB - SPIE

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Koman V, Suárez G, Santschi C, Cadarso VJ, Brugger J, Von Moos N et al. A portable microfluidic-based biophotonic sensor for extracellular H 2O2 measurements. In Advanced Biomedical and Clinical Diagnostic Systems XI. Vol. 8572. SPIE. 2013. 857218 https://doi.org/10.1117/12.2008329