Abstract
Objectives: Noradrenaline released from sympathetic nerves is rapidly inactivated via the action of the noradrenaline transporter (NET). We aimed to determine whether a single nucleotide polymorphism (SNP) in the NET gene, rs7194256, was associated with blood pressure and plasma noradrenaline concentration in patients with resistant hypertension. Methods: Ninety-two consecutive patients with resistant hypertension participated in this study (age 62±1.3 years, BMI 32±0.6kg/m 2, mean±SEM). Blood pressure was assessed using 24-h ambulatory blood pressure monitoring. Genotyping of rs7194256 (C/T) was performed using a predeveloped TaqMan SNP Genotyping Assay. Plasma catecholamines were analyzed using high-performance liquid chromatography. Results: There were no differences in anthropometric measures between those carrying a T allele or the CC genotype. Patients carrying a T allele had significantly higher SBP: 24-h mean 148±2.6 vs. 140±2.4; 24-h max 189±3.2 vs. 179±2.6; 24-h min 114±3.0 vs. 105±2.3; night mean 141±3.0 vs. 131±2.5; night max 170±3.6 vs. 159±3.1; night min 118±3.4 vs. 109±2.4 (all P<0.05). T-allele carriers had a significantly higher arterial noradrenaline concentration: 573±53 vs. 377±35pg/ml (P=0.002) and lower ratio of the intraneuronal noradrenaline metabolite, 3,4-dihydroxyphenylglycol, to noradrenaline (3.01±0.4 vs. 4.08±0.3pg/ml; P=0.024). Conclusion: A SNP in the NET gene in patients with resistant hypertension is associated with higher plasma noradrenaline concentration and elevated SBP. Impaired NET function may be a contributor to the pronounced activation of the sympathetic nervous system characteristic of patients with resistant hypertension.
| Original language | English |
|---|---|
| Pages (from-to) | 1571-1577 |
| Number of pages | 7 |
| Journal | Journal of Hypertension |
| Volume | 36 |
| Issue number | 7 |
| DOIs | |
| Publication status | Published - 1 Jul 2018 |
Keywords
- blood pressure
- noradrenaline
- noradrenaline transporter
- resistant hypertension
- single nucleotide polymorphism
Cite this
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A polymorphism in the noradrenaline transporter gene is associated with increased blood pressure in patients with resistant hypertension. / Eikelis, Nina; Marques, Francine Z.; Hering, Dagmara; Marusic, Petra; Head, Geoffrey A.; Walton, Antony S.; Lambert, Elisabeth A.; Esler, Murray D.; Sari, Carolina I.; Schlaich, Markus P.; Lambert, Gavin W.
In: Journal of Hypertension, Vol. 36, No. 7, 01.07.2018, p. 1571-1577.Research output: Contribution to journal › Article › Research › peer-review
TY - JOUR
T1 - A polymorphism in the noradrenaline transporter gene is associated with increased blood pressure in patients with resistant hypertension
AU - Eikelis, Nina
AU - Marques, Francine Z.
AU - Hering, Dagmara
AU - Marusic, Petra
AU - Head, Geoffrey A.
AU - Walton, Antony S.
AU - Lambert, Elisabeth A.
AU - Esler, Murray D.
AU - Sari, Carolina I.
AU - Schlaich, Markus P.
AU - Lambert, Gavin W.
PY - 2018/7/1
Y1 - 2018/7/1
N2 - Objectives: Noradrenaline released from sympathetic nerves is rapidly inactivated via the action of the noradrenaline transporter (NET). We aimed to determine whether a single nucleotide polymorphism (SNP) in the NET gene, rs7194256, was associated with blood pressure and plasma noradrenaline concentration in patients with resistant hypertension. Methods: Ninety-two consecutive patients with resistant hypertension participated in this study (age 62±1.3 years, BMI 32±0.6kg/m 2, mean±SEM). Blood pressure was assessed using 24-h ambulatory blood pressure monitoring. Genotyping of rs7194256 (C/T) was performed using a predeveloped TaqMan SNP Genotyping Assay. Plasma catecholamines were analyzed using high-performance liquid chromatography. Results: There were no differences in anthropometric measures between those carrying a T allele or the CC genotype. Patients carrying a T allele had significantly higher SBP: 24-h mean 148±2.6 vs. 140±2.4; 24-h max 189±3.2 vs. 179±2.6; 24-h min 114±3.0 vs. 105±2.3; night mean 141±3.0 vs. 131±2.5; night max 170±3.6 vs. 159±3.1; night min 118±3.4 vs. 109±2.4 (all P<0.05). T-allele carriers had a significantly higher arterial noradrenaline concentration: 573±53 vs. 377±35pg/ml (P=0.002) and lower ratio of the intraneuronal noradrenaline metabolite, 3,4-dihydroxyphenylglycol, to noradrenaline (3.01±0.4 vs. 4.08±0.3pg/ml; P=0.024). Conclusion: A SNP in the NET gene in patients with resistant hypertension is associated with higher plasma noradrenaline concentration and elevated SBP. Impaired NET function may be a contributor to the pronounced activation of the sympathetic nervous system characteristic of patients with resistant hypertension.
AB - Objectives: Noradrenaline released from sympathetic nerves is rapidly inactivated via the action of the noradrenaline transporter (NET). We aimed to determine whether a single nucleotide polymorphism (SNP) in the NET gene, rs7194256, was associated with blood pressure and plasma noradrenaline concentration in patients with resistant hypertension. Methods: Ninety-two consecutive patients with resistant hypertension participated in this study (age 62±1.3 years, BMI 32±0.6kg/m 2, mean±SEM). Blood pressure was assessed using 24-h ambulatory blood pressure monitoring. Genotyping of rs7194256 (C/T) was performed using a predeveloped TaqMan SNP Genotyping Assay. Plasma catecholamines were analyzed using high-performance liquid chromatography. Results: There were no differences in anthropometric measures between those carrying a T allele or the CC genotype. Patients carrying a T allele had significantly higher SBP: 24-h mean 148±2.6 vs. 140±2.4; 24-h max 189±3.2 vs. 179±2.6; 24-h min 114±3.0 vs. 105±2.3; night mean 141±3.0 vs. 131±2.5; night max 170±3.6 vs. 159±3.1; night min 118±3.4 vs. 109±2.4 (all P<0.05). T-allele carriers had a significantly higher arterial noradrenaline concentration: 573±53 vs. 377±35pg/ml (P=0.002) and lower ratio of the intraneuronal noradrenaline metabolite, 3,4-dihydroxyphenylglycol, to noradrenaline (3.01±0.4 vs. 4.08±0.3pg/ml; P=0.024). Conclusion: A SNP in the NET gene in patients with resistant hypertension is associated with higher plasma noradrenaline concentration and elevated SBP. Impaired NET function may be a contributor to the pronounced activation of the sympathetic nervous system characteristic of patients with resistant hypertension.
KW - blood pressure
KW - noradrenaline
KW - noradrenaline transporter
KW - resistant hypertension
KW - single nucleotide polymorphism
UR - http://www.scopus.com/inward/record.url?scp=85048310456&partnerID=8YFLogxK
U2 - 10.1097/HJH.0000000000001736
DO - 10.1097/HJH.0000000000001736
M3 - Article
VL - 36
SP - 1571
EP - 1577
JO - Journal of Hypertension
JF - Journal of Hypertension
SN - 0263-6352
IS - 7
ER -