A numerical model of EMT6/Ro spheroid dynamics under irradiation: Calibration and estimation of the underlying irradiation-induced cell survival probability

Simon Angus, Monika Joanna Piotrowska

Research output: Contribution to journalArticleResearchpeer-review

Abstract

We present extensions to our quasi-2D cellular automata spheroid model that add a cellular kinetics module together with an irradiation and repair module. Significantly, our approach is not based on the Linear Quadratic (LQ) model, instead, we propose a simple two-parameter, algorithmic model which captures the essential biological features of irradiation-induced cell death, repair and associated cell cycle delays. This approach allows us to estimate directly the underlying irradiation-induced cell survival probability. We present the calibration of this extended model both with and without the application of single irradiation doses to the commonly studied (in vitro) EMT6/Ro (mammary carcinoma) cell line. A comparison of the estimated underlying cell survival probability with the in vitro survival probability data confirms the expected differences in the measures.
Original languageEnglish
Pages (from-to)23 - 32
Number of pages10
JournalJournal of Theoretical Biology
Volume320
DOIs
Publication statusPublished - 2013

Cite this

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abstract = "We present extensions to our quasi-2D cellular automata spheroid model that add a cellular kinetics module together with an irradiation and repair module. Significantly, our approach is not based on the Linear Quadratic (LQ) model, instead, we propose a simple two-parameter, algorithmic model which captures the essential biological features of irradiation-induced cell death, repair and associated cell cycle delays. This approach allows us to estimate directly the underlying irradiation-induced cell survival probability. We present the calibration of this extended model both with and without the application of single irradiation doses to the commonly studied (in vitro) EMT6/Ro (mammary carcinoma) cell line. A comparison of the estimated underlying cell survival probability with the in vitro survival probability data confirms the expected differences in the measures.",
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A numerical model of EMT6/Ro spheroid dynamics under irradiation: Calibration and estimation of the underlying irradiation-induced cell survival probability. / Angus, Simon; Piotrowska, Monika Joanna.

In: Journal of Theoretical Biology, Vol. 320, 2013, p. 23 - 32.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - A numerical model of EMT6/Ro spheroid dynamics under irradiation: Calibration and estimation of the underlying irradiation-induced cell survival probability

AU - Angus, Simon

AU - Piotrowska, Monika Joanna

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AB - We present extensions to our quasi-2D cellular automata spheroid model that add a cellular kinetics module together with an irradiation and repair module. Significantly, our approach is not based on the Linear Quadratic (LQ) model, instead, we propose a simple two-parameter, algorithmic model which captures the essential biological features of irradiation-induced cell death, repair and associated cell cycle delays. This approach allows us to estimate directly the underlying irradiation-induced cell survival probability. We present the calibration of this extended model both with and without the application of single irradiation doses to the commonly studied (in vitro) EMT6/Ro (mammary carcinoma) cell line. A comparison of the estimated underlying cell survival probability with the in vitro survival probability data confirms the expected differences in the measures.

U2 - 10.1016/j.jtbi.2012.11.035

DO - 10.1016/j.jtbi.2012.11.035

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