A novel two-chain proteinase inhibitor generated by circularization of a multidomain precursor protein

M. C.S. Lee; M. J. Scanlon; D. J. Craik; M. A. Anderson

doi:10.1038/9293

A novel two-chain proteinase inhibitor generated by circularization of a multidomain precursor protein

M. C.S. Lee, M. J. Scanlon, D. J. Craik, M. A. Anderson

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58 Citations (Scopus)

Abstract

Female reproductive tissues of the ornamental tobacco amass high levels of serine proteinase inhibitors (PIs) for protection against pests and pathogens. These PIs are produced from a precursor protein composed of six repeats each with a protease reactive site. Here we show that proteolytic processing of the precursor generates five single-chain PIs and a remarkable two-chain inhibitor formed by disulfide-bond linkage of N- and C-terminal peptide fragments. Surprisingly, PI precursors adopt this circular structure regardless of the number of inhibitor domains, suggesting this bracelet-like conformation is characteristic of the widespread potato inhibitor II (Pot II) protein family.

Original language	English
Pages (from-to)	526-530
Number of pages	5
Journal	Nature Structural Biology
Volume	6
Issue number	6
DOIs	https://doi.org/10.1038/9293
Publication status	Published - 9 Jun 1999
Externally published	Yes

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abstract = "Female reproductive tissues of the ornamental tobacco amass high levels of serine proteinase inhibitors (PIs) for protection against pests and pathogens. These PIs are produced from a precursor protein composed of six repeats each with a protease reactive site. Here we show that proteolytic processing of the precursor generates five single-chain PIs and a remarkable two-chain inhibitor formed by disulfide-bond linkage of N- and C-terminal peptide fragments. Surprisingly, PI precursors adopt this circular structure regardless of the number of inhibitor domains, suggesting this bracelet-like conformation is characteristic of the widespread potato inhibitor II (Pot II) protein family.",

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AU - Craik, D. J.

AU - Anderson, M. A.

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A novel two-chain proteinase inhibitor generated by circularization of a multidomain precursor protein

Abstract

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