A novel tool to identify the relative contribution of lymphoid cell types that contribute to IL-10 production during the infection with Schistosoma mansoni: The TIGER index

Sebastian Scheer, Sandra Gross, Gabriel Mouahid, Hélène Moné, Marinus C. Lamers

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4 Citations (Scopus)


Introduction: Infection with the trematode helminth Schistosoma mansoni affects more than 200 million people worldwide. Infected patients are thought to show a decreased incidence of asthma and autoimmune diseases, which is, among others, considered a result of an increased production of the immunoregulatory cytokine IL-10. However, the location and the type of cell that is responsible for the highest production of IL-10 in vivo are still unknown. Aim: Identification of the hierarchy of IL-10 producing cell types in the mesenteric lymph node and spleen during the course of the murine infection with S. mansoni without the need of an external standard. Methods: We describe the use of the IL-10 reporter mouse TIGER for the study of murine schistosomiasis and introduce a novel tool, which we have called the TIGER index (TI). This index combines data from flow cytometric measurements and cell count analysis and allows identifying the cell type with the highest contribution of IL-10 during the course of infection in the secondary lymphoid organs, sites of extensive immunoregulatory activity in schistosomiasis. Results: In this paper we have calculated the TI for the mesenteric lymph nodes and the spleen in the course of a chronic infection with S. mansoni. Using the TI, we identified CD4pos CD25pos and CD4pos CD25neg cell populations as the highest producers of IL-10 in the mesenteric lymph node and the spleen in chronic schistosomiasis, respectively, whereas B cells, NK cells and NKT cells showed a lower contribution to IL-10 production throughout the infection. Conclusion: The TI is a highly useful tool to measure the relative contribution of different cell types, which are responsible for the in vivo production of IL-10 in the secondary lymphoid organs during the infection with S. mansoni. Thus, the strength of the TI ensures the possibility to analyze IL-10 production in a long term experiment without the need of an external standard between each time point of analysis.

Original languageEnglish
Pages (from-to)66-73
Number of pages8
JournalJournal of Immunological Methods
Publication statusPublished - Apr 2014
Externally publishedYes


  • Flow cytometry
  • Immunoregulation
  • Interleukin 10
  • Reporter mice
  • Schistosoma mansoni

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