A novel linear plasmid mediates flagellar variation in Salmonella Typhi

Stephen Baker, Jonathan Hardy, Kenneth E. Sanderson, Michael Quail, Ian Goodhead, Robert A. Kingsley, Julian Parkhill, Bruce Stocker, Gordon Dougan

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43 Citations (Scopus)

Abstract

Unlike the majority of Salmonella enterica serovars, Salmonella Typhi (S. Typhi), the etiological agent of human typhoid, is monophasic. S. Typhi normally harbours only the phase 1 flagellin gene (fliC), which encodes the H:d antigen. However, some S. Typhi strains found in Indonesia express an additional flagellin antigen termed H:z66. Molecular analysis of H:z66+ S. Typhi revealed that the H:z66 flagellin structural gene (fljBz66) is encoded on a linear plasmid that we have named pBSSB1. The DNA sequence of pBSSB1 was determined to be just over 27 kbp, and was predicted to encode 33 coding sequences. To our knowledge, pBSSB1 is the first non-bacteriophage-related linear plasmid to be described in the Enterobacteriaceae.

Original languageEnglish
Pages (from-to)605-610
Number of pages6
JournalPLoS Pathogens
Volume3
Issue number5
DOIs
Publication statusPublished - 1 Jun 2007
Externally publishedYes

Cite this

Baker, S., Hardy, J., Sanderson, K. E., Quail, M., Goodhead, I., Kingsley, R. A., Parkhill, J., Stocker, B., & Dougan, G. (2007). A novel linear plasmid mediates flagellar variation in Salmonella Typhi. PLoS Pathogens, 3(5), 605-610. https://doi.org/10.1371/journal.ppat.0030059