A novel linear plasmid mediates flagellar variation in Salmonella Typhi

Stephen Baker; Jonathan Hardy; Kenneth E. Sanderson; Michael Quail; Ian Goodhead; Robert A. Kingsley; Julian Parkhill; Bruce Stocker; Gordon Dougan

doi:10.1371/journal.ppat.0030059

A novel linear plasmid mediates flagellar variation in Salmonella Typhi

Stephen Baker, Jonathan Hardy, Kenneth E. Sanderson, Michael Quail, Ian Goodhead, Robert A. Kingsley, Julian Parkhill, Bruce Stocker, Gordon Dougan

Research output: Contribution to journal › Article › Research › peer-review

52 Citations (Scopus)

Abstract

Unlike the majority of Salmonella enterica serovars, Salmonella Typhi (S. Typhi), the etiological agent of human typhoid, is monophasic. S. Typhi normally harbours only the phase 1 flagellin gene (fliC), which encodes the H:d antigen. However, some S. Typhi strains found in Indonesia express an additional flagellin antigen termed H:z66. Molecular analysis of H:z66+ S. Typhi revealed that the H:z66 flagellin structural gene (fljB^z66) is encoded on a linear plasmid that we have named pBSSB1. The DNA sequence of pBSSB1 was determined to be just over 27 kbp, and was predicted to encode 33 coding sequences. To our knowledge, pBSSB1 is the first non-bacteriophage-related linear plasmid to be described in the Enterobacteriaceae.

Original language	English
Pages (from-to)	605-610
Number of pages	6
Journal	PLoS Pathogens
Volume	3
Issue number	5
DOIs	https://doi.org/10.1371/journal.ppat.0030059
Publication status	Published - 1 Jun 2007
Externally published	Yes

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title = "A novel linear plasmid mediates flagellar variation in Salmonella Typhi",

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AU - Baker, Stephen

AU - Hardy, Jonathan

AU - Sanderson, Kenneth E.

AU - Quail, Michael

AU - Goodhead, Ian

AU - Kingsley, Robert A.

AU - Parkhill, Julian

AU - Stocker, Bruce

AU - Dougan, Gordon

PY - 2007/6/1

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N2 - Unlike the majority of Salmonella enterica serovars, Salmonella Typhi (S. Typhi), the etiological agent of human typhoid, is monophasic. S. Typhi normally harbours only the phase 1 flagellin gene (fliC), which encodes the H:d antigen. However, some S. Typhi strains found in Indonesia express an additional flagellin antigen termed H:z66. Molecular analysis of H:z66+ S. Typhi revealed that the H:z66 flagellin structural gene (fljBz66) is encoded on a linear plasmid that we have named pBSSB1. The DNA sequence of pBSSB1 was determined to be just over 27 kbp, and was predicted to encode 33 coding sequences. To our knowledge, pBSSB1 is the first non-bacteriophage-related linear plasmid to be described in the Enterobacteriaceae.

AB - Unlike the majority of Salmonella enterica serovars, Salmonella Typhi (S. Typhi), the etiological agent of human typhoid, is monophasic. S. Typhi normally harbours only the phase 1 flagellin gene (fliC), which encodes the H:d antigen. However, some S. Typhi strains found in Indonesia express an additional flagellin antigen termed H:z66. Molecular analysis of H:z66+ S. Typhi revealed that the H:z66 flagellin structural gene (fljBz66) is encoded on a linear plasmid that we have named pBSSB1. The DNA sequence of pBSSB1 was determined to be just over 27 kbp, and was predicted to encode 33 coding sequences. To our knowledge, pBSSB1 is the first non-bacteriophage-related linear plasmid to be described in the Enterobacteriaceae.

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A novel linear plasmid mediates flagellar variation in Salmonella Typhi

Abstract

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