A novel isoform of the human mitochondrial complex I subunit NDUFV3

Marris G. Dibley, Michael T. Ryan, David A. Stroud

Research output: Contribution to journalLetterResearchpeer-review

Abstract

Human mitochondrial complex I is the first enzyme of the mitochondrial respiratory chain. Complex I is composed of 45 subunits, seven encoded by mitochondrial DNA, while the remainder are encoded by nuclear DNA. All nuclear-encoded subunits are thought to be expressed as a single isoform. Here we reveal subunit NDUFV3 to be present in both the canonical 10 kDa and a novel 50 kDa isoform, generated through alternative splicing. Both isoforms assemble into complex I and their levels vary in different tissues. While the 50 kDa isoform appears to be dominant in HEK293T cells, we find either isoform alone is sufficient for assembly of mature complex I. NDUFV3 represents the first known complex I subunit present in two functional isoforms.

Original languageEnglish
Pages (from-to)109-117
Number of pages9
JournalFEBS Letters
Volume591
Issue number1
DOIs
Publication statusPublished - 1 Jan 2017

Keywords

  • complex I
  • metabolism
  • mitochondria
  • NADH dehydrogenase
  • OXPHOS

Cite this

Dibley, Marris G. ; Ryan, Michael T. ; Stroud, David A. / A novel isoform of the human mitochondrial complex I subunit NDUFV3. In: FEBS Letters. 2017 ; Vol. 591, No. 1. pp. 109-117.
@article{de3b06d6779a49aa8b5f41dedb71eeb0,
title = "A novel isoform of the human mitochondrial complex I subunit NDUFV3",
abstract = "Human mitochondrial complex I is the first enzyme of the mitochondrial respiratory chain. Complex I is composed of 45 subunits, seven encoded by mitochondrial DNA, while the remainder are encoded by nuclear DNA. All nuclear-encoded subunits are thought to be expressed as a single isoform. Here we reveal subunit NDUFV3 to be present in both the canonical 10 kDa and a novel 50 kDa isoform, generated through alternative splicing. Both isoforms assemble into complex I and their levels vary in different tissues. While the 50 kDa isoform appears to be dominant in HEK293T cells, we find either isoform alone is sufficient for assembly of mature complex I. NDUFV3 represents the first known complex I subunit present in two functional isoforms.",
keywords = "complex I, metabolism, mitochondria, NADH dehydrogenase, OXPHOS",
author = "Dibley, {Marris G.} and Ryan, {Michael T.} and Stroud, {David A.}",
year = "2017",
month = "1",
day = "1",
doi = "10.1002/1873-3468.12527",
language = "English",
volume = "591",
pages = "109--117",
journal = "FEBS Letters",
issn = "0014-5793",
publisher = "Wiley-Blackwell",
number = "1",

}

A novel isoform of the human mitochondrial complex I subunit NDUFV3. / Dibley, Marris G.; Ryan, Michael T.; Stroud, David A.

In: FEBS Letters, Vol. 591, No. 1, 01.01.2017, p. 109-117.

Research output: Contribution to journalLetterResearchpeer-review

TY - JOUR

T1 - A novel isoform of the human mitochondrial complex I subunit NDUFV3

AU - Dibley, Marris G.

AU - Ryan, Michael T.

AU - Stroud, David A.

PY - 2017/1/1

Y1 - 2017/1/1

N2 - Human mitochondrial complex I is the first enzyme of the mitochondrial respiratory chain. Complex I is composed of 45 subunits, seven encoded by mitochondrial DNA, while the remainder are encoded by nuclear DNA. All nuclear-encoded subunits are thought to be expressed as a single isoform. Here we reveal subunit NDUFV3 to be present in both the canonical 10 kDa and a novel 50 kDa isoform, generated through alternative splicing. Both isoforms assemble into complex I and their levels vary in different tissues. While the 50 kDa isoform appears to be dominant in HEK293T cells, we find either isoform alone is sufficient for assembly of mature complex I. NDUFV3 represents the first known complex I subunit present in two functional isoforms.

AB - Human mitochondrial complex I is the first enzyme of the mitochondrial respiratory chain. Complex I is composed of 45 subunits, seven encoded by mitochondrial DNA, while the remainder are encoded by nuclear DNA. All nuclear-encoded subunits are thought to be expressed as a single isoform. Here we reveal subunit NDUFV3 to be present in both the canonical 10 kDa and a novel 50 kDa isoform, generated through alternative splicing. Both isoforms assemble into complex I and their levels vary in different tissues. While the 50 kDa isoform appears to be dominant in HEK293T cells, we find either isoform alone is sufficient for assembly of mature complex I. NDUFV3 represents the first known complex I subunit present in two functional isoforms.

KW - complex I

KW - metabolism

KW - mitochondria

KW - NADH dehydrogenase

KW - OXPHOS

UR - http://www.scopus.com/inward/record.url?scp=85009247748&partnerID=8YFLogxK

U2 - 10.1002/1873-3468.12527

DO - 10.1002/1873-3468.12527

M3 - Letter

VL - 591

SP - 109

EP - 117

JO - FEBS Letters

JF - FEBS Letters

SN - 0014-5793

IS - 1

ER -