A novel grass pollen allergen mimotope identified by phage display peptide library inhibits allergen-human IgE antibody interaction

Cenk Suphioglu, George Schäppi, Josephine Kenrick, David Levy, Janet M. Davies, Robyn E. O'Hehir

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24 Citations (Scopus)

Abstract

The aim of this study was to investigate the molecular basis of human IgE-allergen interaction by screening a phage-displayed peptide library with an allergen-specific human IgE-mimicking monoclonal antibody (mAb). A mAb that reacted with major grass pollen allergens was successfully identified and shown to inhibit human IgE-allergen interaction. Biopanning of a phage-displayed random peptide library with this mAb yielded a 12 amino acid long mimotope. A synthetic peptide based on this 12-mer mimotope inhibited mAb and human IgE binding to grass pollen extracts. Our results indicate that such synthetic peptide mimotopes of allergens have potential as novel therapeutic agents.

Original languageEnglish
Pages (from-to)46-52
Number of pages7
JournalFEBS Letters
Volume502
Issue number1-2
DOIs
Publication statusPublished - 27 Jul 2001

Keywords

  • Allergen
  • Epitope
  • Grass pollen
  • Human immunoglobulin E
  • Mimotope
  • Phage display

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