Projects per year
Abstract
Objective: To establish a model of human implantation that responds to hormonal stimuli and can differentiate between endometrium from fertile women and those with idiopathic infertility. Design: A trophoblast stem cell (trophectodermal) line (TSC; derived from human pre-implantation embryo) was used to form trophectodermal spheroids (TS). TS attachment to monolayers of endometrial epithelial cell lines or primary endometrial epithelial cells (pHEECs) was determined. Setting: Independent Medical Research Institute with close clinical linkages Interventions: Spheroid attachment and outgrowth was determined with added hormones (estradiol 17β (E), E + medroxyprogesterone acetate (MPA) or E + MPA + human chorionic gonadotropin (hCG)). Spheroid attachment to E/MPA treated pHEEC prepared from fertile women or those with idiopathic infertility tested. Main outcome measure: Firmly attached spheroids counted after co-culture for 6 h. Outgrowth was determined by quantitation of area covered by spheroid after firm adhesion. Results: Functional adhesion of TS to two endometrial epithelial cell lines, Ishikawa and ECC-1 cells, was hormonally responsive, with adhesion/outgrowth increased by E/MPA (ECC-1; p < 0.01, Ishikawa; p < 0.01) and E/MPA/hCG (ECC-1; p < 0.001, Ishikawa p < 0.01) versus E alone. The same pattern of hormone responsiveness was observed in pHEEC obtained from fertile women (E vs, E/MPA; p < 0.01, E vs. E/MPA/hCG; p < 0.001). TS adhered to 85% of pHEEC obtained from fertile women (11/13) and 11% of pHEEC obtained from women with unexplained infertility (2/18, p < 0.001). Conclusion: This new model of “embryo” implantation largely discriminates between endometrial epithelial cells obtained from fertile vs. infertile women based on adhesion; this holds potential as an in vitro “diagnostic” tool of endometrial infertility.
Original language | English |
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Pages (from-to) | 5-16 |
Number of pages | 12 |
Journal | Journal of Assisted Reproduction and Genetics |
Volume | 37 |
Issue number | 1 |
DOIs | |
Publication status | Published - Jan 2020 |
Projects
- 2 Finished
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Reappraisal of the mechanisms underlying implantation success or failure
Salamonsen, L. (Primary Chief Investigator (PCI)), Greening, D. W. (Chief Investigator (CI)), Evans, J. (Chief Investigator (CI)), Simpson, R. J. (Chief Investigator (CI)), Catt, S. (Chief Investigator (CI)) & Vollenhoven, B. (Chief Investigator (CI))
1/01/18 → 30/06/21
Project: Research
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Amniotic Exosomes - Nanomedicine for Bronchopulmonary Dysplasia
Lim, R. (Primary Chief Investigator (PCI)), Salamonsen, L. (Chief Investigator (CI)), Greening, D. W. (Chief Investigator (CI)), Clevers, H. C. (Chief Investigator (CI)) & Kim, C. F. (Chief Investigator (CI))
NHMRC - National Health and Medical Research Council (Australia)
1/01/18 → 31/12/21
Project: Research