Abstract
The biosensor was constructed for determination of glucose by using glucose oxidase enzyme immobilized on poly(thiophene-3-boronic acid) (PTBA). Boronic acid functionalized polythiophene layer was obtained by electrochemical polymerization of Thiophene (Th) and thiophene-3-boronic acid (TBA) with different monomer rations. The reconstitution of the apo-glucose oxidase (apo-GOx) on a complexed flavin adenine dinucleotide (FAD) linked to polythiophene boronic acid (PTBA) monolayer yields an electrically contacted enzyme monolayer. The GOx-reconstituted enzyme electrode exhibited excellent electrocatalytic activities toward the reduction and oxidation of hydrogen peroxide as well. The PTBA/FAD/GOx biosensor shows an excellent performance for glucose at +0.4 V with a high sensitivity (2.14 μA/mM) and lower response time (∼5 s) in a wide concentration range of 0.5-18 mM (correlation coefficient of 0.9952). Furthermore, the effects of applied potential, pH, temperature, electroactive interference, stability and reusability of the biosensors were discussed.
Original language | English |
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Pages (from-to) | 1199-1204 |
Number of pages | 6 |
Journal | Current Applied Physics |
Volume | 13 |
Issue number | 7 |
DOIs | |
Publication status | Published - 2013 |
Externally published | Yes |
Keywords
- Biosensor
- Boronic acid
- Glucose oxidase
- Polythiophene