A novel amperometric glucose biosensor based on reconstitution of glucose oxidase on thiophene-3-boronic acid polymer layer

Mehmet Şenel, Muamer Dervisevic, Emre Çevik

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17 Citations (Scopus)

Abstract

The biosensor was constructed for determination of glucose by using glucose oxidase enzyme immobilized on poly(thiophene-3-boronic acid) (PTBA). Boronic acid functionalized polythiophene layer was obtained by electrochemical polymerization of Thiophene (Th) and thiophene-3-boronic acid (TBA) with different monomer rations. The reconstitution of the apo-glucose oxidase (apo-GOx) on a complexed flavin adenine dinucleotide (FAD) linked to polythiophene boronic acid (PTBA) monolayer yields an electrically contacted enzyme monolayer. The GOx-reconstituted enzyme electrode exhibited excellent electrocatalytic activities toward the reduction and oxidation of hydrogen peroxide as well. The PTBA/FAD/GOx biosensor shows an excellent performance for glucose at +0.4 V with a high sensitivity (2.14 μA/mM) and lower response time (∼5 s) in a wide concentration range of 0.5-18 mM (correlation coefficient of 0.9952). Furthermore, the effects of applied potential, pH, temperature, electroactive interference, stability and reusability of the biosensors were discussed.

Original languageEnglish
Pages (from-to)1199-1204
Number of pages6
JournalCurrent Applied Physics
Volume13
Issue number7
DOIs
Publication statusPublished - 2013
Externally publishedYes

Keywords

  • Biosensor
  • Boronic acid
  • Glucose oxidase
  • Polythiophene

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