A NIMA-related protein kinase is essential for completion of the sexual cycle of malaria parasites

Luc Reininger, Oliver Billker, Rita Tewari, Arunima Mukhopadhyay, Clare Fennell, Dominique Dorin-Semblat, Caroline Doerig, Dean Goldring, Leonie Harmse, Lisa Ranford-Cartwright, Jeremy Packer, Christian Doerig

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112 Citations (Scopus)

Abstract

The molecular mechanisms regulating the sexual development of malaria parasites from gametocytes to oocysts in their mosquito vector are still largely unexplored. In other eukaryotes, NIMA-related kinases (Neks) regulate cell cycle progression and have been implicated in the regulation of meiosis. Here, we demonstrate that Nek-4, a new Plasmodium member of the Nek family, is essential for completion of the sexual cycle of the parasite. Recombinant Plasmodium falciparum Nek-4 possesses protein kinase activity and displays substrate preferences similar to those of other Neks. Nek-4 is highly expressed in gametocytes, yet disruption of the nek-4 gene in the rodent malaria parasite P. berghei has no effect on gamete formation and subsequent fertilization. However, further differentiation of zygotes into ookinetes is abolished. Measurements of nuclear DNA content indicate that zygotes lacking Nek-4 fail to undergo the genome replication to the tetraploid level that precedes meiosis. Cell cycle progression in the zygote is identified as a likely precondition for its morphological transition to the ookinete and for the successful establishment of a malaria infection in the mosquito.

Original languageEnglish
Pages (from-to)31957-31964
Number of pages8
JournalJournal of Biological Chemistry
Volume280
Issue number36
DOIs
Publication statusPublished - 9 Sep 2005
Externally publishedYes

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