@inbook{01944f98e1204632864ace62c763dda3,
title = "A new, multiplex, quantitative real-time polymerase chain reaction system for nucleic acid detection and quantification",
abstract = "Quantitative real-time polymerase chain reaction (qPCR) has emerged as a powerful investigative and diagnostic tool with potential to generate accurate and reproducible results. qPCR can be designed to fulfil the four key aspects required for the detection of nucleic acids: simplicity, speed, sensitivity, and specificity.This chapter reports the development of a novel real-time multiplex quantitative PCR technology, dubbed PrimRglo{\texttrademark}, with a potential for high-degree multiplexing. It combines the capacity to simultaneously detect many viruses, bacteria, or nucleic acids, in a single reaction tube, with the ability to quantitate viral or bacterial load. The system utilizes oligonucleotide-tagged PCR primers, along with complementary fluorophore-labelled and quencher-labelled oligonucleotides. The analytic sensitivity of PrimRglo technology was compared with the widely used Taqman{\textregistered} and SYBR green detection systems.",
keywords = "Quantitative PCR, Multiplexed PCR, Primer design, PrimRglo, Nucleic acid",
author = "Fang Liang and Neetika Arora and Zhang, {Kang Liang} and Yeh, {David Che Cheng} and Richard Lai and Darnley Pearson and Graeme Barnett and Whiley, {David M} and Theo Sloots and Corrie, {Simon Robert} and Barnard, {Ross Thomas}",
year = "2013",
month = jul,
day = "2",
doi = "10.1007/978-1-62703-535-4_4",
language = "English",
isbn = "9781627035347",
series = "Methods in Molecular Biology",
publisher = "Humana Press",
pages = "51 -- 68",
editor = "Kolpashchikov, {Dmitry M.} and Gerasimova, {Yulia V.}",
booktitle = "Nucleic Acid Detection",
address = "United States of America",
edition = "1",
}