Projects per year
Abstract
A classical view of blood cell development is that multipotent hematopoietic stem and progenitor cells (HSPCs) become lineage-restricted at defined stages. Lin−c-Kit+Sca-1+Flt3+ cells, termed lymphoid-primed multipotent progenitors (LMPPs), have lost megakaryocyte and erythroid potential but are heterogeneous in their fate. Here, through single-cell RNA sequencing, we identify the expression of Dach1 and associated genes in this fraction as being coexpressed with myeloid/stem genes but inversely correlated with lymphoid genes. Through generation of Dach1–GFP reporter mice, we identify a transcriptionally and functionally unique Dach1–GFP− subpopulation within LMPPs with lymphoid potential with low to negligible classic myeloid potential. We term these ‘lymphoid-primed progenitors’ (LPPs). These findings define an early definitive branch point of lymphoid development in hematopoiesis and a means for prospective isolation of LPPs.
Original language | English |
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Pages (from-to) | 1574-1584 |
Number of pages | 11 |
Journal | Nature Immunology |
Volume | 21 |
Issue number | 12 |
DOIs | |
Publication status | Published - Dec 2020 |
Projects
- 2 Finished
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The role of the homeobox transcription factor Hhex in haematopoiesis and leukaemia
McCormack, M.
11/07/16 → 31/12/18
Project: Research
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Development of a system to detect disease outbreaks in semi-isolated communities using automated monitoring of electronic data
19/04/11 → 19/08/11
Project: Research