A new class of amphiphiles bearing rigid hydrophobic groups for solubilization and stabilization of membrane proteins

Pil Seok Chae, Søren G F Rasmussen, Rohini R. Rana, Kamil Gotfryd, Andrew C. Kruse, Aashish Manglik, Kyung Ho Cho, Shailika Nurva, Ulrik Gether, Lan Guan, Claus J. Loland, Bernadette Byrne, Brian K. Kobilka, Samuel H. Gellman

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76 Citations (Scopus)


Non-traditional amphiphiles: Conferring aqueous solubility on membrane proteins generally requires the use of a detergent or other amphiphilic agent. A new class of amphiphiles was synthesized, based on steroidal lipophilic groups, and evaluated with several membrane proteins. The results show that the new amphiphile, "glyco-diosgenin" (GDN; see figure), confers enhanced stability to a variety of membrane proteins in solution relative to popular conventional detergents, such as dodecylmaltoside (DDM).

Original languageEnglish
Pages (from-to)9485-9490
Number of pages6
JournalChemistry - A European Journal
Issue number31
Publication statusPublished - 27 Jul 2012
Externally publishedYes


  • amphiphiles
  • membrane proteins
  • molecular design
  • solubilization
  • stabilization

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