A new class of amphiphiles bearing rigid hydrophobic groups for solubilization and stabilization of membrane proteins

Pil Seok Chae, Søren G F Rasmussen, Rohini R. Rana, Kamil Gotfryd, Andrew C. Kruse, Aashish Manglik, Kyung Ho Cho, Shailika Nurva, Ulrik Gether, Lan Guan, Claus J. Loland, Bernadette Byrne, Brian K. Kobilka, Samuel H. Gellman

Research output: Contribution to journalArticleResearchpeer-review

76 Citations (Scopus)

Abstract

Non-traditional amphiphiles: Conferring aqueous solubility on membrane proteins generally requires the use of a detergent or other amphiphilic agent. A new class of amphiphiles was synthesized, based on steroidal lipophilic groups, and evaluated with several membrane proteins. The results show that the new amphiphile, "glyco-diosgenin" (GDN; see figure), confers enhanced stability to a variety of membrane proteins in solution relative to popular conventional detergents, such as dodecylmaltoside (DDM).

Original languageEnglish
Pages (from-to)9485-9490
Number of pages6
JournalChemistry - A European Journal
Volume18
Issue number31
DOIs
Publication statusPublished - 27 Jul 2012
Externally publishedYes

Keywords

  • amphiphiles
  • membrane proteins
  • molecular design
  • solubilization
  • stabilization

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