A new class of amphiphiles bearing rigid hydrophobic groups for solubilization and stabilization of membrane proteins

Pil Seok Chae; Søren G F Rasmussen; Rohini R. Rana; Kamil Gotfryd; Andrew C. Kruse; Aashish Manglik; Kyung Ho Cho; Shailika Nurva; Ulrik Gether; Lan Guan; Claus J. Loland; Bernadette Byrne; Brian K. Kobilka; Samuel H. Gellman

doi:10.1002/chem.201200069

A new class of amphiphiles bearing rigid hydrophobic groups for solubilization and stabilization of membrane proteins

Pil Seok Chae, Søren G F Rasmussen, Rohini R. Rana, Kamil Gotfryd, Andrew C. Kruse, Aashish Manglik, Kyung Ho Cho, Shailika Nurva, Ulrik Gether, Lan Guan, Claus J. Loland, Bernadette Byrne, Brian K. Kobilka, Samuel H. Gellman

Research output: Contribution to journal › Article › Research › peer-review

128 Citations (Scopus)

Abstract

Non-traditional amphiphiles: Conferring aqueous solubility on membrane proteins generally requires the use of a detergent or other amphiphilic agent. A new class of amphiphiles was synthesized, based on steroidal lipophilic groups, and evaluated with several membrane proteins. The results show that the new amphiphile, "glyco-diosgenin" (GDN; see figure), confers enhanced stability to a variety of membrane proteins in solution relative to popular conventional detergents, such as dodecylmaltoside (DDM).

Original language	English
Pages (from-to)	9485-9490
Number of pages	6
Journal	Chemistry - A European Journal
Volume	18
Issue number	31
DOIs	https://doi.org/10.1002/chem.201200069
Publication status	Published - 27 Jul 2012
Externally published	Yes

Keywords

amphiphiles
membrane proteins
molecular design
solubilization
stabilization

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10.1002/chem.201200069

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Chae, P. S., Rasmussen, S. G. F., Rana, R. R., Gotfryd, K., Kruse, A. C., Manglik, A., Cho, K. H., Nurva, S., Gether, U., Guan, L., Loland, C. J., Byrne, B., Kobilka, B. K., & Gellman, S. H. (2012). A new class of amphiphiles bearing rigid hydrophobic groups for solubilization and stabilization of membrane proteins. Chemistry - A European Journal, 18(31), 9485-9490. https://doi.org/10.1002/chem.201200069

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abstract = "Non-traditional amphiphiles: Conferring aqueous solubility on membrane proteins generally requires the use of a detergent or other amphiphilic agent. A new class of amphiphiles was synthesized, based on steroidal lipophilic groups, and evaluated with several membrane proteins. The results show that the new amphiphile, {"}glyco-diosgenin{"} (GDN; see figure), confers enhanced stability to a variety of membrane proteins in solution relative to popular conventional detergents, such as dodecylmaltoside (DDM).",

keywords = "amphiphiles, membrane proteins, molecular design, solubilization, stabilization",

author = "Chae, {Pil Seok} and Rasmussen, {S{\o}ren G F} and Rana, {Rohini R.} and Kamil Gotfryd and Kruse, {Andrew C.} and Aashish Manglik and Cho, {Kyung Ho} and Shailika Nurva and Ulrik Gether and Lan Guan and Loland, {Claus J.} and Bernadette Byrne and Kobilka, {Brian K.} and Gellman, {Samuel H.}",

year = "2012",

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Chae, PS, Rasmussen, SGF, Rana, RR, Gotfryd, K, Kruse, AC, Manglik, A, Cho, KH, Nurva, S, Gether, U, Guan, L, Loland, CJ, Byrne, B, Kobilka, BK & Gellman, SH 2012, 'A new class of amphiphiles bearing rigid hydrophobic groups for solubilization and stabilization of membrane proteins', Chemistry - A European Journal, vol. 18, no. 31, pp. 9485-9490. https://doi.org/10.1002/chem.201200069

TY - JOUR

T1 - A new class of amphiphiles bearing rigid hydrophobic groups for solubilization and stabilization of membrane proteins

AU - Chae, Pil Seok

AU - Rasmussen, Søren G F

AU - Rana, Rohini R.

AU - Gotfryd, Kamil

AU - Kruse, Andrew C.

AU - Manglik, Aashish

AU - Cho, Kyung Ho

AU - Nurva, Shailika

AU - Gether, Ulrik

AU - Guan, Lan

AU - Loland, Claus J.

AU - Byrne, Bernadette

AU - Kobilka, Brian K.

AU - Gellman, Samuel H.

PY - 2012/7/27

Y1 - 2012/7/27

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AB - Non-traditional amphiphiles: Conferring aqueous solubility on membrane proteins generally requires the use of a detergent or other amphiphilic agent. A new class of amphiphiles was synthesized, based on steroidal lipophilic groups, and evaluated with several membrane proteins. The results show that the new amphiphile, "glyco-diosgenin" (GDN; see figure), confers enhanced stability to a variety of membrane proteins in solution relative to popular conventional detergents, such as dodecylmaltoside (DDM).

KW - amphiphiles

KW - membrane proteins

KW - molecular design

KW - solubilization

KW - stabilization

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SN - 0947-6539

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SP - 9485

EP - 9490

JO - Chemistry - A European Journal

JF - Chemistry - A European Journal

IS - 31

ER -

A new class of amphiphiles bearing rigid hydrophobic groups for solubilization and stabilization of membrane proteins

Abstract

Keywords

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