A naturally occurring peptide with an elementary single disulfide-directed β-hairpin fold

Samuel Robinson, Sandeep Chhabra, Alessia Belgi, Balasubramanyam Chittoor, Helena Safavi-Hemami, Andrea J Robinson, Anthony T. Papenfuss, Anthony Purcell, Raymond Norton

Research output: Contribution to journalArticleResearchpeer-review

Abstract

Certain peptide folds, owing to a combination of intrinsic stability and resilience to amino acid substitutions, are particularly effective for the display of diverse functional groups. Such "privileged scaffolds" are valuable as starting points for the engineering of new bioactive molecules. We have identified a precursor peptide expressed in the venom gland of the marine snail Conus victoriae, which appears to belong to a hitherto undescribed class of molluscan neuropeptides. Mass spectrometry matching with the venom confirmed the complete mature peptide sequence as a 31-residue peptide with a single disulfide bond. Solution structure determination revealed a unique peptide fold that we have designated the single disulfide-directed β hairpin (SDH). The SDH fold is highly resistant to thermal denaturation and forms the core of several other multiple disulfide-containing peptide folds, including the inhibitor cystine knot. This elementary fold may offer a valuable starting point for the design and engineering of new bioactive peptides.

Original languageEnglish
Pages (from-to)293-299
Number of pages7
JournalStructure
Volume24
Issue number2
DOIs
Publication statusPublished - 2 Feb 2016

Cite this

Robinson, Samuel ; Chhabra, Sandeep ; Belgi, Alessia ; Chittoor, Balasubramanyam ; Safavi-Hemami, Helena ; Robinson, Andrea J ; Papenfuss, Anthony T. ; Purcell, Anthony ; Norton, Raymond. / A naturally occurring peptide with an elementary single disulfide-directed β-hairpin fold. In: Structure. 2016 ; Vol. 24, No. 2. pp. 293-299.
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Robinson, S, Chhabra, S, Belgi, A, Chittoor, B, Safavi-Hemami, H, Robinson, AJ, Papenfuss, AT, Purcell, A & Norton, R 2016, 'A naturally occurring peptide with an elementary single disulfide-directed β-hairpin fold' Structure, vol. 24, no. 2, pp. 293-299. https://doi.org/10.1016/j.str.2015.11.015

A naturally occurring peptide with an elementary single disulfide-directed β-hairpin fold. / Robinson, Samuel; Chhabra, Sandeep; Belgi, Alessia; Chittoor, Balasubramanyam; Safavi-Hemami, Helena; Robinson, Andrea J; Papenfuss, Anthony T.; Purcell, Anthony; Norton, Raymond.

In: Structure, Vol. 24, No. 2, 02.02.2016, p. 293-299.

Research output: Contribution to journalArticleResearchpeer-review

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