A native Wolbachia endosymbiont does not limit dengue virus infection in the mosquito Aedes notoscriptus (Diptera: Culicidae)

Ellie Skelton, Edwige Rancès, Francesca D. Frentiu, Endang Srimurni Kusmintarsih, Iñaki Iturbe-Ormaetxe, Eric P. Caragata, Megan Woolfit, Scott L. O'Neill

Research output: Contribution to journalArticleResearchpeer-review

Abstract

The endosymbiotic bacterium Wolbachia pipientis infects many species of insects and has been transinfected into the mosquito Aedes aegypti (L.), the primary vector of dengue virus (DENV). Recently, it has been shown that Wolbachia blocks the replication and transmission of RNA viruses, such as DENV, in a number of mosquito species including Ae. aegypti and Aedes albopictus (Skuse), which is naturally infected with Wolbachia and considered a secondary vector for DENV. The mosquito species Aedes notoscriptus (Skuse) is highly prevalent in Australia, including in areas where DENV outbreaks have been recorded. The mosquito has been implicated in the transmission of Ross River and Barmah Forest viruses, but not DENV. We investigated whether Wolbachia naturally infects this mosquito species and whether it has an impact on the ability of Ae. notoscriptus to transmit DENV. We show, for the first time, that Ae. notoscriptus is naturally infected with a strain of Wolbachia that belongs to supergroup B and is localized only in the ovaries. However, Wolbachia infection in Ae. notoscriptus did not induce resistance to DENV and had no effect on overall DENV infection rate or titer. The presence of a native Wolbachia in Ae. notoscriptus cannot explain why this mosquito is an ineffective vector of DENV.

Original languageEnglish
Pages (from-to)401-408
Number of pages8
JournalJournal of Medical Entomology
Volume53
Issue number2
DOIs
Publication statusPublished - 1 Mar 2016

Keywords

  • Aedes notoscriptus
  • Dengue virus
  • Tissue tropism
  • Vector competence
  • Wolbachia pipientis

Cite this

Skelton, Ellie ; Rancès, Edwige ; Frentiu, Francesca D. ; Kusmintarsih, Endang Srimurni ; Iturbe-Ormaetxe, Iñaki ; Caragata, Eric P. ; Woolfit, Megan ; O'Neill, Scott L. / A native Wolbachia endosymbiont does not limit dengue virus infection in the mosquito Aedes notoscriptus (Diptera : Culicidae). In: Journal of Medical Entomology. 2016 ; Vol. 53, No. 2. pp. 401-408.
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abstract = "The endosymbiotic bacterium Wolbachia pipientis infects many species of insects and has been transinfected into the mosquito Aedes aegypti (L.), the primary vector of dengue virus (DENV). Recently, it has been shown that Wolbachia blocks the replication and transmission of RNA viruses, such as DENV, in a number of mosquito species including Ae. aegypti and Aedes albopictus (Skuse), which is naturally infected with Wolbachia and considered a secondary vector for DENV. The mosquito species Aedes notoscriptus (Skuse) is highly prevalent in Australia, including in areas where DENV outbreaks have been recorded. The mosquito has been implicated in the transmission of Ross River and Barmah Forest viruses, but not DENV. We investigated whether Wolbachia naturally infects this mosquito species and whether it has an impact on the ability of Ae. notoscriptus to transmit DENV. We show, for the first time, that Ae. notoscriptus is naturally infected with a strain of Wolbachia that belongs to supergroup B and is localized only in the ovaries. However, Wolbachia infection in Ae. notoscriptus did not induce resistance to DENV and had no effect on overall DENV infection rate or titer. The presence of a native Wolbachia in Ae. notoscriptus cannot explain why this mosquito is an ineffective vector of DENV.",
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Skelton, E, Rancès, E, Frentiu, FD, Kusmintarsih, ES, Iturbe-Ormaetxe, I, Caragata, EP, Woolfit, M & O'Neill, SL 2016, 'A native Wolbachia endosymbiont does not limit dengue virus infection in the mosquito Aedes notoscriptus (Diptera: Culicidae)' Journal of Medical Entomology, vol. 53, no. 2, pp. 401-408. https://doi.org/10.1093/jme/tjv235

A native Wolbachia endosymbiont does not limit dengue virus infection in the mosquito Aedes notoscriptus (Diptera : Culicidae). / Skelton, Ellie; Rancès, Edwige; Frentiu, Francesca D.; Kusmintarsih, Endang Srimurni; Iturbe-Ormaetxe, Iñaki; Caragata, Eric P.; Woolfit, Megan; O'Neill, Scott L.

In: Journal of Medical Entomology, Vol. 53, No. 2, 01.03.2016, p. 401-408.

Research output: Contribution to journalArticleResearchpeer-review

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