A murine respiratory-inducing niche displays variable efficiency across human and mouse embryonic stem cell species

Mark Denham; Brock James Conley; Fredrik Par Olsson; Lerna Gulluyan; Timothy James Cole; Richard Anthony Mollard

doi:10.1152/ajplung.00440.2006

A murine respiratory-inducing niche displays variable efficiency across human and mouse embryonic stem cell species

Mark Denham, Brock James Conley, Fredrik Par Olsson, Lerna Gulluyan, Timothy James Cole, Richard Anthony Mollard

Research output: Contribution to journal › Article › Research › peer-review

8 Citations (Scopus)

Abstract

Human embryonic stemlike cells (hESCs) are pluripotent cells derived from blastocysts. Differentiating hESCs into respiratory lineages may benefit respiratory therapeutic programs. We previously demonstrated that 24 of all mouse embryonic stem cell (mESC) derivatives cocultured with embryonic day 11.5 (E11.5) mouse lung rudiments display immunoreactivity to the pneumonocyte II specific marker surfactant-associated protein C (Sftpc). Here we further investigate the effects of this inductive niche in terms of its competence to induce hESC derivative SFTPC immunoreactivity and the expression of other markers of terminal lung secretory units. When hESCs were cocultured as single cells, clumps of approximately 10 cells or embryoid bodies (EBs), hESC derivatives formed pan-keratin-positive epithelial tubules at high frequency (>30 of all hESC derivatives). However, human-specific SFTPC immunoreactivity associated with tubule formation only at low frequency (

Original language	English
Pages (from-to)	1241 - 1247
Number of pages	7
Journal	American Journal of Physiology - Lung Cellular and Molecular Physiology
Volume	292
Issue number	5
DOIs	https://doi.org/10.1152/ajplung.00440.2006
Publication status	Published - 2007

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10.1152/ajplung.00440.2006

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title = "A murine respiratory-inducing niche displays variable efficiency across human and mouse embryonic stem cell species",

abstract = "Human embryonic stemlike cells (hESCs) are pluripotent cells derived from blastocysts. Differentiating hESCs into respiratory lineages may benefit respiratory therapeutic programs. We previously demonstrated that 24 of all mouse embryonic stem cell (mESC) derivatives cocultured with embryonic day 11.5 (E11.5) mouse lung rudiments display immunoreactivity to the pneumonocyte II specific marker surfactant-associated protein C (Sftpc). Here we further investigate the effects of this inductive niche in terms of its competence to induce hESC derivative SFTPC immunoreactivity and the expression of other markers of terminal lung secretory units. When hESCs were cocultured as single cells, clumps of approximately 10 cells or embryoid bodies (EBs), hESC derivatives formed pan-keratin-positive epithelial tubules at high frequency (>30 of all hESC derivatives). However, human-specific SFTPC immunoreactivity associated with tubule formation only at low frequency (",

author = "Mark Denham and Conley, {Brock James} and Olsson, {Fredrik Par} and Lerna Gulluyan and Cole, {Timothy James} and Mollard, {Richard Anthony}",

year = "2007",

doi = "10.1152/ajplung.00440.2006",

language = "English",

volume = "292",

pages = "1241 -- 1247",

journal = "American Journal of Physiology - Lung Cellular and Molecular Physiology",

issn = "1040-0605",

publisher = "American Physiological Society",

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A murine respiratory-inducing niche displays variable efficiency across human and mouse embryonic stem cell species. / Denham, Mark; Conley, Brock James; Olsson, Fredrik Par et al.
In: American Journal of Physiology - Lung Cellular and Molecular Physiology, Vol. 292, No. 5, 2007, p. 1241 - 1247.

Research output: Contribution to journal › Article › Research › peer-review

TY - JOUR

T1 - A murine respiratory-inducing niche displays variable efficiency across human and mouse embryonic stem cell species

AU - Denham, Mark

AU - Conley, Brock James

AU - Olsson, Fredrik Par

AU - Gulluyan, Lerna

AU - Cole, Timothy James

AU - Mollard, Richard Anthony

PY - 2007

Y1 - 2007

N2 - Human embryonic stemlike cells (hESCs) are pluripotent cells derived from blastocysts. Differentiating hESCs into respiratory lineages may benefit respiratory therapeutic programs. We previously demonstrated that 24 of all mouse embryonic stem cell (mESC) derivatives cocultured with embryonic day 11.5 (E11.5) mouse lung rudiments display immunoreactivity to the pneumonocyte II specific marker surfactant-associated protein C (Sftpc). Here we further investigate the effects of this inductive niche in terms of its competence to induce hESC derivative SFTPC immunoreactivity and the expression of other markers of terminal lung secretory units. When hESCs were cocultured as single cells, clumps of approximately 10 cells or embryoid bodies (EBs), hESC derivatives formed pan-keratin-positive epithelial tubules at high frequency (>30 of all hESC derivatives). However, human-specific SFTPC immunoreactivity associated with tubule formation only at low frequency (

AB - Human embryonic stemlike cells (hESCs) are pluripotent cells derived from blastocysts. Differentiating hESCs into respiratory lineages may benefit respiratory therapeutic programs. We previously demonstrated that 24 of all mouse embryonic stem cell (mESC) derivatives cocultured with embryonic day 11.5 (E11.5) mouse lung rudiments display immunoreactivity to the pneumonocyte II specific marker surfactant-associated protein C (Sftpc). Here we further investigate the effects of this inductive niche in terms of its competence to induce hESC derivative SFTPC immunoreactivity and the expression of other markers of terminal lung secretory units. When hESCs were cocultured as single cells, clumps of approximately 10 cells or embryoid bodies (EBs), hESC derivatives formed pan-keratin-positive epithelial tubules at high frequency (>30 of all hESC derivatives). However, human-specific SFTPC immunoreactivity associated with tubule formation only at low frequency (

UR - http://ajplung.physiology.org/cgi/reprint/292/5/L1241

U2 - 10.1152/ajplung.00440.2006

DO - 10.1152/ajplung.00440.2006

M3 - Article

SN - 1040-0605

VL - 292

SP - 1241

EP - 1247

JO - American Journal of Physiology - Lung Cellular and Molecular Physiology

JF - American Journal of Physiology - Lung Cellular and Molecular Physiology

IS - 5

ER -

A murine respiratory-inducing niche displays variable efficiency across human and mouse embryonic stem cell species

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