A murine respiratory-inducing niche displays variable efficiency across human and mouse embryonic stem cell species

Mark Denham, Brock James Conley, Fredrik Par Olsson, Lerna Gulluyan, Timothy James Cole, Richard Anthony Mollard

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8 Citations (Scopus)


Human embryonic stemlike cells (hESCs) are pluripotent cells derived from blastocysts. Differentiating hESCs into respiratory lineages may benefit respiratory therapeutic programs. We previously demonstrated that 24 of all mouse embryonic stem cell (mESC) derivatives cocultured with embryonic day 11.5 (E11.5) mouse lung rudiments display immunoreactivity to the pneumonocyte II specific marker surfactant-associated protein C (Sftpc). Here we further investigate the effects of this inductive niche in terms of its competence to induce hESC derivative SFTPC immunoreactivity and the expression of other markers of terminal lung secretory units. When hESCs were cocultured as single cells, clumps of approximately 10 cells or embryoid bodies (EBs), hESC derivatives formed pan-keratin-positive epithelial tubules at high frequency (>30 of all hESC derivatives). However, human-specific SFTPC immunoreactivity associated with tubule formation only at low frequency (
Original languageEnglish
Pages (from-to)1241 - 1247
Number of pages7
JournalAmerican Journal of Physiology - Lung Cellular and Molecular Physiology
Issue number5
Publication statusPublished - 2007

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