A multimode-TIRFM and microfluidic technique to examine platelet adhesion dynamics

Warwick Scott Nesbitt, Francisco J Tovar-Lopez, Erik Westein, Ian Steward Harper, Shaun Jackson

Research output: Chapter in Book/Report/Conference proceedingChapter (Book)Researchpeer-review

5 Citations (Scopus)


Fluorescence microscopy techniques have provided important insights into the structural and signalling events occurring during platelet adhesion under both static and blood flow conditions. However, due to limitations in sectioning ability and sensitivity these techniques are restricted in their capacity to precisely image the adhesion footprint of spreading platelets. In particular, investigation of platelet adhesion under hemodynamic shear stress requires an imaging platform with high spatial discrimination and sensitivity and rapid temporal resolution. This chapter describes in detail a multimode imaging approach combining total internal reflection fluorescence microscopy (TIRFM) with high speed epifluorescence and differential interference contrast (DIC) microscopy along with a novel microfluidic perfusion system developed in our laboratory to examine platelet membrane adhesion dynamics under static and flow conditions. ? Springer Science+Business Media, LLC 2013.
Original languageEnglish
Title of host publicationAdhesion Protein Protocols
EditorsAmanda S Coutts
Place of PublicationUSA
PublisherHumana Press
Pages39 - 58
Number of pages20
ISBN (Print)9781627035385
Publication statusPublished - 2013

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