A mouse model of hereditary coproporphyria identified in an ENU mutagenesis screen

Ashlee J. Conway, Fiona C. Brown, Robert O. Fullinfaw, Benjamin T. Kile, Stephen M. Jane, David J. Curtis

Research output: Contribution to journalArticleResearchpeer-review

2 Citations (Scopus)

Abstract

A genome-wide ethyl-N-nitrosourea (ENU) mutagenesis screen in mice was performed to identify novel regulators of erythropoiesis. Here, we describe a mouse line, RBC16, which harbours a dominantly inherited mutation in the Cpox gene, responsible for production of the haem biosynthesis enzyme, coproporphyrinogen III oxidase (CPOX). A premature stop codon in place of a tryptophan at amino acid 373 results in reduced mRNA expression and diminished protein levels, yielding a microcytic red blood cell phenotype in heterozygous mice. Urinary and faecal porphyrins in female RBC16 heterozygotes were significantly elevated compared with that of wildtype littermates, particularly coproporphyrinogen III, whereas males were biochemically normal. Attempts to induce acute porphyric crises were made using fasting and phenobarbital treatment on females. While fasting hadnobiochemical effect on RBC16 mice, phenobarbital caused significant elevation of faecal coproporphyrinogen III in heterozygous mice. This is the first known investigation of a mutagenesis mouse model with genetic and biochemical parallels to hereditary coproporphyria.

Original languageEnglish
Pages (from-to)1005-1013
Number of pages9
JournalDisease Models and Mechanisms
Volume10
Issue number8
DOIs
Publication statusPublished - 1 Aug 2017

Keywords

  • Anaemia
  • CPOX
  • Ethyl-N-nitrosourea
  • Hereditary coproporphyria

Cite this

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abstract = "A genome-wide ethyl-N-nitrosourea (ENU) mutagenesis screen in mice was performed to identify novel regulators of erythropoiesis. Here, we describe a mouse line, RBC16, which harbours a dominantly inherited mutation in the Cpox gene, responsible for production of the haem biosynthesis enzyme, coproporphyrinogen III oxidase (CPOX). A premature stop codon in place of a tryptophan at amino acid 373 results in reduced mRNA expression and diminished protein levels, yielding a microcytic red blood cell phenotype in heterozygous mice. Urinary and faecal porphyrins in female RBC16 heterozygotes were significantly elevated compared with that of wildtype littermates, particularly coproporphyrinogen III, whereas males were biochemically normal. Attempts to induce acute porphyric crises were made using fasting and phenobarbital treatment on females. While fasting hadnobiochemical effect on RBC16 mice, phenobarbital caused significant elevation of faecal coproporphyrinogen III in heterozygous mice. This is the first known investigation of a mutagenesis mouse model with genetic and biochemical parallels to hereditary coproporphyria.",
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A mouse model of hereditary coproporphyria identified in an ENU mutagenesis screen. / Conway, Ashlee J.; Brown, Fiona C.; Fullinfaw, Robert O.; Kile, Benjamin T.; Jane, Stephen M.; Curtis, David J.

In: Disease Models and Mechanisms, Vol. 10, No. 8, 01.08.2017, p. 1005-1013.

Research output: Contribution to journalArticleResearchpeer-review

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