A monoclonal antibody to aromatic amino acid hydroxylases. Identification of the epitope

R. G.H. Cotton, W. McAdam, I. Jennings, F. J. Morgan

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Abstract

PH8 monoclonal antibody has previously been shown to react with all three aromatic amino acid hydroxylases, being particularly useful for immunohistochemical staining of brain tissue [Haan, Jennings, Cuello, Nakata, Chow, Kushinsky, Brittingham & Cotton (1987) Brain Res. 426, 19-27]. Western-blot analysis of liver extracts showed that PH8 reacted with phenylalanine hydroxylase from a wide range of vertebrate species. The epitope for antibody PH8 has been localized to the human phenylalanine hydroxylase sequence between amino acid residues 139 and 155. This highly conserved region of the aromatic amino acid hydroxylases has 11 out of 17 amino acids identical in phenylalanine hydroxylase, tyrosine hydroxylase and tryptophan hydroxylase.

Original languageEnglish
Pages (from-to)193-196
Number of pages4
JournalBiochemical Journal
Volume255
Issue number1
DOIs
Publication statusPublished - 1 Jan 1988
Externally publishedYes

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