A molecular beacon approach to measuring the DNA permeability of thin films

Angus P R Johnston, Frank Caruso

Research output: Contribution to journalArticleResearchpeer-review

35 Citations (Scopus)

Abstract

A new method for determining the permeability of thin films has been developed. A molecular beacon immobilized inside a porous silica particle that is subsequently encapsulated within a thin film can be used to determine the size of DNA that can permeate through the film. Using this technique, it has been determined that over 3 h, molecules larger than 4.7 nm do not permeate 15-nm thick polyelectrolyte multilayers and after 75 h molecules larger than 6 nm were excluded. This technique has applications for determining the permeability of films used for controlled drug and gene delivery.

Original languageEnglish
Pages (from-to)10014-10015
Number of pages2
JournalJournal of the American Chemical Society
Volume127
Issue number28
DOIs
Publication statusPublished - 20 Jul 2005
Externally publishedYes

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