A molecular basis for the T cell response in HLA-DQ2.2 mediated celiac disease

Yi Tian Ting, Shiva Dahal-Koirala, Hui Shi Keshia Kim, Shuo Wang Qiao, Ralf S. Neumann, Knut E.A. Lundin, Jan Petersen, Hugh H. Reid, Ludvig M. Sollid, Jamie Rossjohn

Research output: Contribution to journalArticleResearchpeer-review

21 Citations (Scopus)


The highly homologous human leukocyte antigen (HLA)-DQ2 molecules, HLA-DQ2.5 and HLA-DQ2.2, are implicated in the pathogenesis of celiac disease (CeD) by presenting gluten peptides to CD4+ T cells. However, while HLA-DQ2.5 is strongly associated with disease, HLA-DQ2.2 is not, and the molecular basis underpinning this differential disease association is unresolved. We here provide structural evidence for how the single polymorphic residue (HLA-DQ2.5-Tyr22α and HLA-DQ2.2-Phe22α) accounts for HLADQ2.2 additionally requiring gluten epitopes possessing a serine at the P3 position of the peptide. In marked contrast to the biased T cell receptor (TCR) usage associated with HLA-DQ2.5–mediated CeD, we demonstrate with extensive single-cell sequencing that a diverse TCR repertoire enables recognition of the immunodominant HLA-DQ2.2-glut-L1 epitope. The crystal structure of two CeD patient-derived TCR in complex with HLA-DQ2.2 and DQ2.2-glut-L1 (PFSEQEQPV) revealed a docking strategy, and associated interatomic contacts, which was notably distinct from the structures of the TCR:HLA-DQ2.5:gliadin epitope complexes. Accordingly, while the molecular surfaces of the antigen-binding clefts of HLA-DQ2.5 and HLA-DQ2.2 are very similar, differences in the nature of the peptides presented translates to differences in responding T cell repertoires and the nature of engagement of the respective antigen-presenting molecules, which ultimately is associated with differing disease penetrance.

Original languageEnglish
Pages (from-to)3063-3073
Number of pages11
JournalProceedings of the National Academy of Sciences of the United States of America
Issue number6
Publication statusPublished - 11 Feb 2020


  • Celiac disease
  • Human leukocyte antigen
  • T cell receptor
  • X-ray crystallography

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