Abstract
Zebrafish larvae are suitable in vivo models for toxicological and pharmacological screens due to their transparency, small size, ex utero development, and genetic and physiological similarity to humans. Using modern imaging techniques, cells and tissues can be dynamically visualized over several days in multiple zebrafish larvae. However, precise specimen immobilization and maintenance of homeostatic conditions remain a challenge for longitudinal studies. A highly customizable mounting configuration with inbuilt means of controlling temperature and media flow would therefore be a valuable tool to facilitate long-term imaging of a large number of specimens. Using three-dimensional printing, we have developed a millifluidic, modular homeostatic imaging plate (HIP), which consists of a customizable sample insert and a temperature-controlled incubation chamber that is continuously perfused, providing an ideal environment for long-term experiments where homeostatic conditions are desired. The HIP is cheap to produce, has a standard microtiter well plate format, and can be fitted to most microscopes. We used the device to image dynamic regeneration of spinal cord neurons. The flexibility and adaptability of the HIP facilitate long-term in vivo imaging of many samples, and can be easily adapted to suit a broad range of specimens.
| Original language | English |
|---|---|
| Pages (from-to) | 37-46 |
| Number of pages | 10 |
| Journal | Zebrafish |
| Volume | 16 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - 31 Jan 2019 |
Keywords
- 3D printing
- bioassay
- imaging
- millifluidics
- regeneration
- zebrafish
Cite this
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A modular millifluidic homeostatic imaging plate for imaging of larval zebrafish. / Friedrich, Timo; Douek, Alon M.; Vandestadt, Celia; Wlodkowic, Donald; Kaslin, Jan.
In: Zebrafish, Vol. 16, No. 1, 31.01.2019, p. 37-46.Research output: Contribution to journal › Article › Research › peer-review
TY - JOUR
T1 - A modular millifluidic homeostatic imaging plate for imaging of larval zebrafish
AU - Friedrich, Timo
AU - Douek, Alon M.
AU - Vandestadt, Celia
AU - Wlodkowic, Donald
AU - Kaslin, Jan
PY - 2019/1/31
Y1 - 2019/1/31
N2 - Zebrafish larvae are suitable in vivo models for toxicological and pharmacological screens due to their transparency, small size, ex utero development, and genetic and physiological similarity to humans. Using modern imaging techniques, cells and tissues can be dynamically visualized over several days in multiple zebrafish larvae. However, precise specimen immobilization and maintenance of homeostatic conditions remain a challenge for longitudinal studies. A highly customizable mounting configuration with inbuilt means of controlling temperature and media flow would therefore be a valuable tool to facilitate long-term imaging of a large number of specimens. Using three-dimensional printing, we have developed a millifluidic, modular homeostatic imaging plate (HIP), which consists of a customizable sample insert and a temperature-controlled incubation chamber that is continuously perfused, providing an ideal environment for long-term experiments where homeostatic conditions are desired. The HIP is cheap to produce, has a standard microtiter well plate format, and can be fitted to most microscopes. We used the device to image dynamic regeneration of spinal cord neurons. The flexibility and adaptability of the HIP facilitate long-term in vivo imaging of many samples, and can be easily adapted to suit a broad range of specimens.
AB - Zebrafish larvae are suitable in vivo models for toxicological and pharmacological screens due to their transparency, small size, ex utero development, and genetic and physiological similarity to humans. Using modern imaging techniques, cells and tissues can be dynamically visualized over several days in multiple zebrafish larvae. However, precise specimen immobilization and maintenance of homeostatic conditions remain a challenge for longitudinal studies. A highly customizable mounting configuration with inbuilt means of controlling temperature and media flow would therefore be a valuable tool to facilitate long-term imaging of a large number of specimens. Using three-dimensional printing, we have developed a millifluidic, modular homeostatic imaging plate (HIP), which consists of a customizable sample insert and a temperature-controlled incubation chamber that is continuously perfused, providing an ideal environment for long-term experiments where homeostatic conditions are desired. The HIP is cheap to produce, has a standard microtiter well plate format, and can be fitted to most microscopes. We used the device to image dynamic regeneration of spinal cord neurons. The flexibility and adaptability of the HIP facilitate long-term in vivo imaging of many samples, and can be easily adapted to suit a broad range of specimens.
KW - 3D printing
KW - bioassay
KW - imaging
KW - millifluidics
KW - regeneration
KW - zebrafish
UR - http://www.scopus.com/inward/record.url?scp=85060987700&partnerID=8YFLogxK
U2 - 10.1089/zeb.2018.1684
DO - 10.1089/zeb.2018.1684
M3 - Article
VL - 16
SP - 37
EP - 46
JO - Zebrafish
JF - Zebrafish
SN - 1545-8547
IS - 1
ER -