A modular BAM complex in the outer membrane of the alpha-proteobacterium caulobacter crescentus

Khatira Anwari; Sebastian Poggio; Andrew Perry; Xenia Gatsos; Sri Harsha Ramarathinam; Nicholas A Williamson; Nicholas Noinaj; Susan Buchanan; Kip Gabriel; Anthony W Purcell; Christine Jacobs-Wagner; Trevor Lithgow

doi:10.1371/journal.pone.0008619

A modular BAM complex in the outer membrane of the alpha-proteobacterium caulobacter crescentus

Khatira Anwari, Sebastian Poggio, Andrew Perry, Xenia Gatsos, Sri Harsha Ramarathinam, Nicholas A Williamson, Nicholas Noinaj, Susan Buchanan, Kip Gabriel, Anthony W Purcell, Christine Jacobs-Wagner, Trevor Lithgow

Biochemistry & Molecular Biology

Research output: Contribution to journal › Article › Research › peer-review

56 Citations (Scopus)

Abstract

Mitochondria are organelles derived from an intracellular alpha-proteobacterium. The biogenesis of mitochondria relies on the assembly of beta-barrel proteins into the mitochondrial outer membrane, a process inherited from the bacterial ancestor. Caulobacter crescentus is an alpha-proteobacterium, and the BAM (beta-barrel assembly machinery) complex was purified and characterized from this model organism. Like the mitochondrial sorting and assembly machinery complex, we find the BAM complex to be modular in nature. A approximately 150 kDa core BAM complex containing BamA, BamB, BamD, and BamE associates with additional modules in the outer membrane. One of these modules, Pal, is a lipoprotein that provides a means for anchorage to the peptidoglycan layer of the cell wall. We suggest the modular design of the BAM complex facilitates access to substrates from the protein translocase in the inner membrane.

Original language	English
Article number	e8619
Number of pages	8
Journal	PLoS ONE
Volume	5
Issue number	1
DOIs	https://doi.org/10.1371/journal.pone.0008619
Publication status	Published - 2010

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10.1371/journal.pone.0008619

Cite this

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title = "A modular BAM complex in the outer membrane of the alpha-proteobacterium caulobacter crescentus",

abstract = "Mitochondria are organelles derived from an intracellular alpha-proteobacterium. The biogenesis of mitochondria relies on the assembly of beta-barrel proteins into the mitochondrial outer membrane, a process inherited from the bacterial ancestor. Caulobacter crescentus is an alpha-proteobacterium, and the BAM (beta-barrel assembly machinery) complex was purified and characterized from this model organism. Like the mitochondrial sorting and assembly machinery complex, we find the BAM complex to be modular in nature. A approximately 150 kDa core BAM complex containing BamA, BamB, BamD, and BamE associates with additional modules in the outer membrane. One of these modules, Pal, is a lipoprotein that provides a means for anchorage to the peptidoglycan layer of the cell wall. We suggest the modular design of the BAM complex facilitates access to substrates from the protein translocase in the inner membrane.",

author = "Khatira Anwari and Sebastian Poggio and Andrew Perry and Xenia Gatsos and Ramarathinam, {Sri Harsha} and Williamson, {Nicholas A} and Nicholas Noinaj and Susan Buchanan and Kip Gabriel and Purcell, {Anthony W} and Christine Jacobs-Wagner and Trevor Lithgow",

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T1 - A modular BAM complex in the outer membrane of the alpha-proteobacterium caulobacter crescentus

AU - Anwari, Khatira

AU - Poggio, Sebastian

AU - Perry, Andrew

AU - Gatsos, Xenia

AU - Ramarathinam, Sri Harsha

AU - Williamson, Nicholas A

AU - Noinaj, Nicholas

AU - Buchanan, Susan

AU - Gabriel, Kip

AU - Purcell, Anthony W

AU - Jacobs-Wagner, Christine

AU - Lithgow, Trevor

PY - 2010

Y1 - 2010

N2 - Mitochondria are organelles derived from an intracellular alpha-proteobacterium. The biogenesis of mitochondria relies on the assembly of beta-barrel proteins into the mitochondrial outer membrane, a process inherited from the bacterial ancestor. Caulobacter crescentus is an alpha-proteobacterium, and the BAM (beta-barrel assembly machinery) complex was purified and characterized from this model organism. Like the mitochondrial sorting and assembly machinery complex, we find the BAM complex to be modular in nature. A approximately 150 kDa core BAM complex containing BamA, BamB, BamD, and BamE associates with additional modules in the outer membrane. One of these modules, Pal, is a lipoprotein that provides a means for anchorage to the peptidoglycan layer of the cell wall. We suggest the modular design of the BAM complex facilitates access to substrates from the protein translocase in the inner membrane.

AB - Mitochondria are organelles derived from an intracellular alpha-proteobacterium. The biogenesis of mitochondria relies on the assembly of beta-barrel proteins into the mitochondrial outer membrane, a process inherited from the bacterial ancestor. Caulobacter crescentus is an alpha-proteobacterium, and the BAM (beta-barrel assembly machinery) complex was purified and characterized from this model organism. Like the mitochondrial sorting and assembly machinery complex, we find the BAM complex to be modular in nature. A approximately 150 kDa core BAM complex containing BamA, BamB, BamD, and BamE associates with additional modules in the outer membrane. One of these modules, Pal, is a lipoprotein that provides a means for anchorage to the peptidoglycan layer of the cell wall. We suggest the modular design of the BAM complex facilitates access to substrates from the protein translocase in the inner membrane.

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DO - 10.1371/journal.pone.0008619

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