A method for the incremental expansion of polyglutamine repeats in recombinant proteins

Amy Louise Robertson, Stephen Paul Bottomley

Research output: Contribution to journalArticleResearchpeer-review


The polyglutamine diseases are caused by the expansion of CAG repeats. A key step in understanding the disease mechanisms, at the DNA and protein level, is the ability to produce recombinant proteins with specific length glutamine tracts which is a time-consuming first step in setting up in vitro systems to study the effects of polyglutamine expansion. Described here is a PCR-based method for the amplification of CAG repeats, which we used to incrementally extend CAG length by 3-5 repeats per cycle. This method could be translated into various contexts where amplification of repeating elements is necessary.
Original languageEnglish
Pages (from-to)73-83
Number of pages11
JournalMethods in Molecular Biology
Publication statusPublished - 2013

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