A Method for the Establishment of Human Lung Adenocarcinoma Patient-Derived Xenografts in Mice

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Abstract

Patient-derived xenografts (PDXs) are created by implanting human tumor tissue or cells into immunodeficent mice, and enable the study of tumor biology, biomarkers and response to therapy in vivo. This chapter describes a method for lung adenocarcinoma (LAC) PDX generation using subcutaneous implantation of tumor tissue and cell suspensions and incorporating the humanization of PDX models by reconstitution with human immune cells.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
EditorsPedro G. Santiago-Cardona
Place of PublicationNew York NY USA
PublisherHumana Press
Chapter13
Pages165-173
Number of pages9
ISBN (Electronic)9781071612781
ISBN (Print)9781071612774
DOIs
Publication statusPublished - 2021

Publication series

NameMethods in Molecular Biology
PublisherHumana Press
Volume2279
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Keywords

  • Humanization
  • Lung adenocarcinoma
  • NOD scid gamma mouse
  • Non-small cell lung cancer
  • Patient-derived xenograft

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