TY - JOUR
T1 - A Lymphostromal Molecule, Thymic Shared Ag-1, Regulates Early Thymocyte Development in Fetal Thymus Organ Culture
AU - Randle-Barrett, Elise
AU - Waanders, Gary A.
AU - Masciantonio, Marilina
AU - Godfrey, Dale I.
AU - Boyd, Richard L.
PY - 1993/12/1
Y1 - 1993/12/1
N2 - Previously, we detailed the characterization of thymic shared Ag-1, a unique marker of immature thymocytes and isolated thymic stromal cells, defined by the mAb MTS 35. In this study, the functional relevance of this molecule to thymopoiesis was investigated by the addition of purified MTS 35 to fetal thymus organ culture. It down-regulated thymic shared Ag-1 expression and dramatically reduced thymocyte cell yield through inhibition of αβ-TcR+ T cell differentiation, post CD3-CD4-CD8- triple negative thymocytes. These effects were specific for the mAb MTS 35, because controls, which include both isotype-matched and other lymphostromal mAb, showed no similar effects. These results demonstrate that thymic shared Ag-1 is a functionally important marker of early thymocyte differentiation, particularly with regard to the αβ-TcR lineage.
AB - Previously, we detailed the characterization of thymic shared Ag-1, a unique marker of immature thymocytes and isolated thymic stromal cells, defined by the mAb MTS 35. In this study, the functional relevance of this molecule to thymopoiesis was investigated by the addition of purified MTS 35 to fetal thymus organ culture. It down-regulated thymic shared Ag-1 expression and dramatically reduced thymocyte cell yield through inhibition of αβ-TcR+ T cell differentiation, post CD3-CD4-CD8- triple negative thymocytes. These effects were specific for the mAb MTS 35, because controls, which include both isotype-matched and other lymphostromal mAb, showed no similar effects. These results demonstrate that thymic shared Ag-1 is a functionally important marker of early thymocyte differentiation, particularly with regard to the αβ-TcR lineage.
UR - http://www.scopus.com/inward/record.url?scp=0027372280&partnerID=8YFLogxK
U2 - 10.4049/jimmunol.151.11.6027
DO - 10.4049/jimmunol.151.11.6027
M3 - Article
C2 - 8245448
AN - SCOPUS:0027372280
SN - 0022-1767
VL - 151
SP - 6027
EP - 6035
JO - Journal of Immunology
JF - Journal of Immunology
IS - 11
ER -