A low-cost, small volume circuit for autologous blood normothermic perfusion of rabbit organs

Murray Worner, Samuel Poore, Daniel Tilkorn, Zerina Lokmic, Anthony J. Penington

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4 Citations (Scopus)

Abstract

We have designed a laboratory extracorporeal normothermic blood perfusion system for whole organs (e.g., kidney) that achieves pulsatile flow, low levels of hemolysis, and a blood priming volume of 60mL or less. Using this uniquely designed extracorporeal circuit, we have achieved perfusion of two isolated ex vivo constructs. In the first experiment, we successfully perfused a rabbit epigastric flap based on the femoral vessels. In the second experiment, we were able to perfuse the isolated rabbit kidney for 48h (range for all kidneys was 12-48h) with excellent urine output, normal arterial blood gasses at 24h, and normal ex vivo kidney histology at the conclusion of the experiments. These parameters have not been achieved before with any known or previously published laboratory extracorporeal circuits. The study has implications for prolonged organ perfusion prior to transplantation and for tissue engineering of vascularized tissues, such as by the perfusion of decellularized organs.

Original languageEnglish
Pages (from-to)352-361
Number of pages10
JournalArtificial Organs
Volume38
Issue number4
DOIs
Publication statusPublished - Apr 2014
Externally publishedYes

Keywords

  • Endothelium
  • Extracorporeal perfusion
  • Normothermic perfusion
  • Transplantation

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