A low-cost pulse generator for exacerbating muscle fiber detachment phenotypes in Zebrafish

Ana Beatriz Delavia Thomasi, Carmen Sonntag, Danilo Fernando Pires, David Zuidema, Antonio Benci, Peter David Currie, Alasdair John Wood

Research output: Contribution to journalArticleResearchpeer-review

Abstract

Muscle fiber detachment from myoseptal boundaries is a common finding in zebrafish models of muscular dystrophies. In some instances, there is a weakening of the interaction between muscle fiber and myosepta, which is yet to manifest as a fiber detachment phenotype. Therefore, to push the fiber detachment of muscle, mutant fish but not their wild-type siblings, beyond their binding threshold, a series of small electrical pulses can be applied to the larvae to create a maximal force contraction and ultimately fiber detachment. To do this, we built a digital pulse generator which delivers four 8 ms 30 V pulses in quick succession, and it has the advantage over older analog approaches to pulse generation because it improves accuracy and is appreciably less expensive. Our pulse generator significantly increases fiber detachment in the laminin-α2 deficient, congenital muscular dystrophy type 1a (MDC1a) model lama2−/− fish when compared with controls.
Original languageEnglish
Pages (from-to)420-424
Number of pages5
JournalZebrafish
Volume15
Issue number4
DOIs
Publication statusPublished - 1 Aug 2018

Keywords

  • muscular dystrophy
  • tendon
  • myotendinous junction
  • laminin
  • MDC1a

Cite this

Thomasi, Ana Beatriz Delavia ; Sonntag, Carmen ; Pires, Danilo Fernando ; Zuidema, David ; Benci, Antonio ; Currie, Peter David ; Wood, Alasdair John. / A low-cost pulse generator for exacerbating muscle fiber detachment phenotypes in Zebrafish. In: Zebrafish. 2018 ; Vol. 15, No. 4. pp. 420-424.
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abstract = "Muscle fiber detachment from myoseptal boundaries is a common finding in zebrafish models of muscular dystrophies. In some instances, there is a weakening of the interaction between muscle fiber and myosepta, which is yet to manifest as a fiber detachment phenotype. Therefore, to push the fiber detachment of muscle, mutant fish but not their wild-type siblings, beyond their binding threshold, a series of small electrical pulses can be applied to the larvae to create a maximal force contraction and ultimately fiber detachment. To do this, we built a digital pulse generator which delivers four 8 ms 30 V pulses in quick succession, and it has the advantage over older analog approaches to pulse generation because it improves accuracy and is appreciably less expensive. Our pulse generator significantly increases fiber detachment in the laminin-α2 deficient, congenital muscular dystrophy type 1a (MDC1a) model lama2−/− fish when compared with controls.",
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A low-cost pulse generator for exacerbating muscle fiber detachment phenotypes in Zebrafish. / Thomasi, Ana Beatriz Delavia; Sonntag, Carmen; Pires, Danilo Fernando; Zuidema, David; Benci, Antonio; Currie, Peter David; Wood, Alasdair John.

In: Zebrafish, Vol. 15, No. 4, 01.08.2018, p. 420-424.

Research output: Contribution to journalArticleResearchpeer-review

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T1 - A low-cost pulse generator for exacerbating muscle fiber detachment phenotypes in Zebrafish

AU - Thomasi, Ana Beatriz Delavia

AU - Sonntag, Carmen

AU - Pires, Danilo Fernando

AU - Zuidema, David

AU - Benci, Antonio

AU - Currie, Peter David

AU - Wood, Alasdair John

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AB - Muscle fiber detachment from myoseptal boundaries is a common finding in zebrafish models of muscular dystrophies. In some instances, there is a weakening of the interaction between muscle fiber and myosepta, which is yet to manifest as a fiber detachment phenotype. Therefore, to push the fiber detachment of muscle, mutant fish but not their wild-type siblings, beyond their binding threshold, a series of small electrical pulses can be applied to the larvae to create a maximal force contraction and ultimately fiber detachment. To do this, we built a digital pulse generator which delivers four 8 ms 30 V pulses in quick succession, and it has the advantage over older analog approaches to pulse generation because it improves accuracy and is appreciably less expensive. Our pulse generator significantly increases fiber detachment in the laminin-α2 deficient, congenital muscular dystrophy type 1a (MDC1a) model lama2−/− fish when compared with controls.

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KW - tendon

KW - myotendinous junction

KW - laminin

KW - MDC1a

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